FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (16): 16-20.doi: 10.7506/spkx1002-6630-201016004

• Processing Technology • Previous Articles     Next Articles

Macroporous Adsorption Resin for the Purification of Flavonoids from Seabuckthorn Pomace

JIAO Yan1,2,WANG Zhen-yu1,3,*   

  1. 1.School of Forestry, Northeast Forestry University, Harbin 150040, China ;
    2.College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China;
    3.College of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China
  • Received:2009-11-24 Online:2010-08-25 Published:2010-12-29
  • Contact: WANG Zhen-yu E-mail:jiaoyan_3000@126.com

Abstract:

Considering overall adsorption and desorption capacity, X-5 resin was selected out of 7 types of macroporous resins to purify the crude flavonoids extract from seabuckthorn pomace. The optimum process conditions for purifying the crude flavonoids extract using X-5 resin were determined as follows: sample concentration 2 mg/mL, adsorption duration 2 h, amount of distilled water used for washing the resin 4 BV, and amount of 70% ethanol as desorption solvent 4 BV. Optimized purification gave a maximum recovery of flavonoids of 86.78% and an increase in the purity from 11.6% to 34.1%. X-5 resin was used repeatedly 6 times for flavonoids purification, and both the recovery and purity of flavonoids remained higher.

Key words: macroporous adsorption resin, purification, seabuckthorn pomace, flavonoids

CLC Number: