Purification and Structural Identification of Chlorogenic Acid from Eucommia ulmoides Leaves

doi:10.7506/spkx1002-6630-201014063

Abstract

Abstract:

Eucommia ulmoides leaves were extracted with 60% aqueous ethanol under the assistance of ultrasound. The crude extract was then condensed on a rotation evaporator, added with chitosan acetic acid solution and stirred, allowed to stand at 60℃ for 60 min for precipitate sedimentation, and filtrated. The filtrate was decolorized with activated carbon prior to 5-time ethyl acetate extraction to remove flavonoids. The lower phase was collected and a chlorpgenic acid extract was obtained. Seven types of moroporous resins were used to purify the chlorpgenic acid extract, and NKA-2 was the selected resin because it exhibited good selectivity to chlorpgenic acid and the static adsorption and desorption ratios were 99.67% and 62.8%. The use of 30% acidified ethanol (pH 3) provided an optimum elution. After NKA-2 purification in the gradient elution mode, a chlorpgenic acid product with 76.3% purity was obtained. Chlorpgenic acid was confirmed by HPLC-MS analysis to be a condensation product of caffeic acid and quinic acid.

Key words: chlorogenic acid, macroporous resin, purification, identification

CLC Number:

LU Qi，DUAN Jia-cai，GAO Li，CHEN Ruo-wen，FU Hong-fei，PAN Si-yi*. Purification and Structural Identification of Chlorogenic Acid from Eucommia ulmoides Leaves[J]. FOOD SCIENCE, 2010, 31(14): 275-279 https://doi.org/10.7506/spkx1002-6630-201014063

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Purification and Structural Identification of Chlorogenic Acid from Eucommia ulmoides Leaves

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