FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (13): 214-218.doi: 10.7506/spkx1002-6630-201013049

• Bioengineering • Previous Articles     Next Articles

Preparation and Identification of Tetrachloroisophthalonethile Artificial Antigen

GUO Nai-fei1,2,GAO Xing1,2,JI Shu-juan1,YU Ji-cheng2,*   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110161, China;
    2. College of Life Science, Dalian Nationalities University, Dalian 116600, China
  • Received:2009-11-12 Online:2010-07-01 Published:2010-12-29
  • Contact: YU Ji-cheng


In this work, tetrachloroisophthalonethile (TCPN) was derivatized into TCPN-COOH through two-step chemical reactions to prepare a hapten. TCPN artificial antigens, TCPN-COOH-BSA and TCPN-COOH-OVA were prepared from the hapten separately conjugated with carrier proteins, i.e., bovine serum albumin (BSA) and ovalbumin (OVA) by activated ester and mixed anhydride methods. An antisera was obtained through the immunization of New Zealand white rabbits using TCPNBSA. Antiserum titer was detected by enzyme-linked immunosorbent assay (ELISA). Meanwhile, TCPN-COOH and its intermediate product TCPN-OH were characterized by IR, MS and NMR methods, and TCPN-COOH-BSA and TCPNCOOH- OVA were qualitatively analyzed by UV/Vis spectrophotometry. TCPN-COOH, TCPN-COOH-BSA and TCPNCOOH- OVA were successfully synthesized, and the coupling ratios between TCPN-COOH and BSA or OVA were 27.86 and 12.32, respectively. The antiserum titer was about 1:1.28 × 104.

Key words: tetrachloroisophthalonethile, hapten, artificial antigen, identification

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