PCR Detection of Escherichia coli O157 in Food and Sequencing of wzy Gene

doi:10.7506/spkx1002-6630-201012044

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (12): 193-196.doi: 10.7506/spkx1002-6630-201012044

• Analysis & Detection • Previous Articles Next Articles

PCR Detection of Escherichia coli O157 in Food and Sequencing of wzy Gene

LI Rui，ZHANG Zhong-mei，DAI Shi-jiao，SUN Yan-feng，HUANG Wan-qi*

College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China

Received:2009-10-21 Online:2010-06-15 Published:2010-12-29
Contact: HUANG Wan-qi E-mail:wanqi2002@yahoo.com.cn

Abstract

Abstract:

A strain of Escherichia coli O157:H7 was isolated from fresh beef and named EC5. EC5 was subjected to PCR amplification and sequencing of full-length wzy gene. There was a 100% similarity between the wzy gene sequence of EC5 and that of Escherichia coli O157:H7 EDL933. Specific primers were designed based on the conserved locus of wzy gene sequences known from NCBI GenBank database for differentiating O157 strains from non-O157 ones by PCR assay. Our findings demonstrate that the wzy gene amplified by PCR is highly specific and provides a promising marker gene for the detection of Escherichia coli O157:H7.

Key words: Escherichia coli O157, PCR detection, wzy gene, sequencing

CLC Number:

R155.5

LI Rui，ZHANG Zhong-mei，DAI Shi-jiao，SUN Yan-feng，HUANG Wan-qi*. PCR Detection of Escherichia coli O157 in Food and Sequencing of wzy Gene[J]. FOOD SCIENCE, 2010, 31(12): 193-196.

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PCR Detection of Escherichia coli O157 in Food and Sequencing of wzy Gene

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