FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (8): 24-27.doi: 10.7506/spkx1002-6300-201008006

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Macroporous Resin Adsorption for Purification of Crude Flavonoids from Bamboo Leaves

YOU Hui,SUN Ai-dong*,TANG Ling,PAN Na,CHEN Jian   

  1. Department of Food Science, College of Biological Sciences and Biotechnology, Beijing Forestry University, Bejing 100083, China
  • Received:2009-01-19 Online:2010-04-15 Published:2010-12-29
  • Contact: SUN Ai-dong*, E-mail:adsun68@163.com

Abstract:

To screen an optimal adsorbent for purification of flavonoids from bamboo leaves, 7 macroporous resins were compared for difference in adsorption and desorption performance. AB-8 resin was chosen due to the merits of large adsorption capacity, easy desorption and high resolution. Besides, the optimal adsorption/desorption process for purification of bamboo leaf flavonoids using AB-8 resin was investigated. Results showed that the optimal pH and flow rate of sample solution were 5.0 and 1 mL/min, respectively and the optimal desorption was achieved by using 4 BV of 60% aqueous ethanol at a flow rate of 1.5 mL/min. Under such conditions, the purity of bamboo leaf flavonoids obtained was up to 54.16%.

Key words: macroporous resins, bamboo leaves, flavonoids, separation, purification

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