In this paper, the present determination methods for β-glucanase activity are reviewed, which includes viscometric method, chromomeric method, agar diffusion method, and commonly used end-reducing methods such as DNS, Somogyi-Nelson and BCA methods, as well as novel MBTH method. In addition, the end-reducing methods are classified as end-point method, multiple-point method, microplate method, and flow injection analytic method according to the operation procedures. Meanwhile, current problems with these methods are discussed, and the future development trends of the determination methods are proposed.