米邦塔仙人掌多糖对SDS 致细胞膜和DNA 损伤的保护作用

doi:10.7506/spkx1002-6630-201011048

食品科学 ›› 2010, Vol. 31 ›› Issue (11): 226-230.doi: 10.7506/spkx1002-6630-201011048

米邦塔仙人掌多糖对SDS 致细胞膜和DNA 损伤的保护作用

王海涛¹，何聪芬¹，董银卯¹，张慧明^1,2，邳楠¹，刘文婷¹，赵华^{1 ,*}

1.北京工商大学北京市植物资源研究开发重点实验室 2.黑龙江中医药大学药学院

收稿日期:2009-10-14 出版日期:2010-06-01 发布日期:2010-12-29
通讯作者: 赵华 E-mail:congfenhe@126.com
基金资助:
国家“863”计划项目(2007AA100404)

Protective Effect of Opuntia milpa alta Polysaccharides on SDS-induced Damage of Human Cell Membrane and Mouse Hepatocyte DNA

WANG Hai-tao1，HE Cong-fen1，DONG Yin-mao1，ZHANG Hui-ming1,2，PI Nan1，LIU Wen-ting1，ZHAO Hua1,*

1. Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing
100048, China；2. Pharmaceutical College, Heilongjiang University of Chinese Medicine, Harbin 150040, China

Received:2009-10-14 Online:2010-06-01 Published:2010-12-29
Contact: ZHAO Hua E-mail:congfenhe@126.com

摘要/Abstract

摘要：

目的：探讨米邦塔仙人掌多糖(OMAP)对十二烷基硫酸钠(SDS)致人血红细胞膜损伤和小鼠肝细胞DNA 损伤的保护作用。方法：分别采用血红细胞(RBC)溶血实验和彗星实验检测细胞膜和DNA 的损伤程度。实验分为3组：阳性对照组，体系中仅加入40μg/mL SDS 致损；OMAP 自溶对照组：体系中仅加入不同质量浓度的OMAP(20、40、60、80、100μg/mL)；OMAP+SDS 组：不同质量浓度OMAP(质量浓度同OMAP 自溶对照组)预孵细胞，再加入40μg/mL SDS 致损。通过测定血红细胞溶血率和致损细胞拖尾率及尾长分别表征细胞膜及DNA 的损伤程度。结果：各剂量OMAP 均能降低SDS 致损细胞膜及DNA 的损伤，且二者呈现一定的剂量- 效应关系。结论：OMAP 对细胞膜和DNA 具有保护作用，可抑制SDS 对细胞膜和DNA 的损伤。

关键词: 米邦塔仙人掌多糖, 红细胞溶血实验, 彗星实验, 细胞膜损伤, DNA 损伤

Abstract:

Objective: To investigate the protective effect of Opuntia milpa alta polysaccharides (OMAP) on human red blood cell (RBC) membrane and mouse hepatocyte DNA damage induced by sodium dodecyl sulfate (SDS). Methods: RBC hemolysis test and Comet assay were used to determine the effect of OMAP on cell membrane damage and DNA damage, respectively. Three groups were designed for the experiments. The positive control group of cell system was given 40μg/mL SDS; OMAP control groups of cell system were cultured in media containing OMAP with varying concentrations (20, 40, 60, 80 μg/mL and 100μg/mL); OMAP + SDS groups of cell system were first treated with OMAP at varying concentrations prior to incubation in media with 40 μg/mL SDS. Results: OMAP presence at all concentration conditions could markedly prevent cell membrane and DNA damage induced by SDS, and an obvious dosage-response relationship was observed. Conclusion: OMAP has good potential to protect SDS-induced cell membrane and DNA damage.

Key words: Opuntia milpa alta polysaccharides, red blood cell hemolysis test, comet assay, cell membrane damage, DNA damage

中图分类号:

Q819

王海涛1，何聪芬1，董银卯1，张慧明1,2，邳楠1，刘文婷1，赵华1,*. 米邦塔仙人掌多糖对SDS 致细胞膜和DNA 损伤的保护作用[J]. 食品科学, 2010, 31(11): 226-230.

WANG Hai-tao1，HE Cong-fen1，DONG Yin-mao1，ZHANG Hui-ming1,2，PI Nan1，LIU Wen-ting1，ZHAO Hua1,*. Protective Effect of Opuntia milpa alta Polysaccharides on SDS-induced Damage of Human Cell Membrane and Mouse Hepatocyte DNA[J]. FOOD SCIENCE, 2010, 31(11): 226-230.

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米邦塔仙人掌多糖对SDS 致细胞膜和DNA 损伤的保护作用

Protective Effect of Opuntia milpa alta Polysaccharides on SDS-induced Damage of Human Cell Membrane and Mouse Hepatocyte DNA

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