响应面分析法优化β-环糊精包合分离花椒籽油中α-亚麻酸的工艺

doi:10.7506/spkx1002-6630-201320010

食品科学

响应面分析法优化β-环糊精包合分离花椒籽油中α-亚麻酸的工艺

薛华丽

甘肃农业大学理学院，甘肃兰州 730070

出版日期:2013-09-25 发布日期:2013-09-27

Application of Response Surface Methodology to Optimize the Separation Technique for α-Linolenic Acid by β-Cyclodetrin Inclusion from Pricklyash Seed Oil

XUE Hua-li

College of Science, Gansu Agricultural University, Lanzhou 730070, China

Online:2013-09-25 Published:2013-09-27

摘要/Abstract

摘要：

在单因素试验的基础上，以水与β-环糊精(β-CD)比例、包合温度、包合时间为自变量，α-亚麻酸的提纯率为响应值，采用Box-Behnken试验设计，利用响应面分析法对花椒籽油中α-亚麻酸的分离工艺进行优化。结果表明：最佳分离工艺条件为：水:β-环糊精(β-CD)=9.9:1、包合温度64℃、包合时间1.32h，此条件下，β-环糊精包合花椒籽油的包合率最高达22.42%。通过红外光谱仪(FT-IR)验证了包合反应的发生，采用气相色谱-质谱联用仪(GC-MS)对包合前后脂肪酸成分进行分析，共检测出3种脂肪酸，使α-亚麻酸的相对含量由4.12%提高到21.35%。

关键词: 花椒籽油, &alpha, -亚麻酸, &beta, -环糊精, 响应面分析

Abstract:

The optimized separation conditions for α-linolenic acid from pricklyash seed oil were determined by using
single factor experiments and response surface analysis. The ratio of water to β-CD, inclusion temperature and inclusion time
were used as the independent variables and the yield of α-linolenic acid was used as the response value. The results showed
that the optimum separation conditions were the ratio of water to β-CD 9.9:1, inclusion temperature 64 ℃ and inclusion
time 1.32 h. The resulting inclusion rate reached 22.42%. The occurrence of inclusion reactions was verified by FT-IR. The
composition of unsaturated fatty acids was analyzed by GC-MS after inclusion reactions, and three fatty acids were retained
in the oil. The content of α-linolenic acid was improved from 4.12% to 21.35%.

Key words: pricklyash seed oil；&alpha, -linolenic acid；&beta, -cyclodetrin；response surface analysis

薛华丽. 响应面分析法优化β-环糊精包合分离花椒籽油中α-亚麻酸的工艺[J]. 食品科学, doi: 10.7506/spkx1002-6630-201320010.

XUE Hua-li. Application of Response Surface Methodology to Optimize the Separation Technique for α-Linolenic Acid by β-Cyclodetrin Inclusion from Pricklyash Seed Oil[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201320010.

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响应面分析法优化β-环糊精包合分离花椒籽油中α-亚麻酸的工艺

Application of Response Surface Methodology to Optimize the Separation Technique for α-Linolenic Acid by β-Cyclodetrin Inclusion from Pricklyash Seed Oil

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