• 工艺技术 •

三相盐析萃取分离辣椒红色素和辣椒碱及不同辣椒品种的聚类分析

1. （1.山西大学生命科学学院，山西 太原 030006；2.特色植物资源研究与利用山西重点实验室（山西大学），山西 太原 030006）
• 出版日期:2020-10-25 发布日期:2020-10-23
• 基金资助:
山西省重点研发计划重点项目（201703D211019；201703D211012-1）；山西省专利推广实施资助专项（20171002）； 山西省“1331工程”山西特色生物资源与健康产业协同创新中心项目（2017）；山西省高等教育机构项目（20181401）

Separation of Capsanthin and Capsaicin by Three-phase Salting-out Extraction and Cluster Analysis of Different Varieties of Chili (Capsicum annuum L.)

FAN Sanhong, WANG Jiaojiao, BAI Baoqing, ZHANG Jinhua

1. (1. School of Life Science, Shanxi University, Taiyuan 030006, China;2. Shanxi Key Laboratory for Research and Development of Regional Plants, Shanxi University, Taiyuan 030006, China)
• Online:2020-10-25 Published:2020-10-23

Abstract: A high performance liquid chromatography (HPLC) method was developed for the determination of capsanthin and capsaicin in chili peppers by using a three-phase salting-out extraction system (petroleum ether/acetonitrile/dipotassium hydrogen phosphate). The composition of the system was determined, and the effects of temperature, sonication time and mixing sequence on the separation of the target products were further investigated. Based on the yields of capsaicin and capsaicin, the optimal extraction conditions were determined by response surface methodology as follows: temperature 45 ℃, sonication time 10 min, distilled water 44%, dipotassium hydrogen phosphate 20%, acetonitrile 20%, and petroleum ether 16%. After the capsicum sample powder was treated by the three-phase mixed solvent, capsanthin and capsaicin were concentrated in the upper and middle phases, respectively, and the concentrations of the target products in either phase were analyzed by HPLC. The results showed that the highest yields of capsanthin and capsaicin under optimal conditions were 0.263 mg/g and 1.412 mg/g, respectively. The proposed method could extract both capsaicin and capsaicin from chili peppers quickly and efficiently. The contents of capsanthin and capsaicin in 33 different varieties of chili peppers were determined, and these varieties were evaluated by cluster analysis, aiming to provide a theoretical basis for the identification and cultivation of chili varieties.