食品科学 ›› 2010, Vol. 31 ›› Issue (4): 103-105.doi: 10.7506/spkx1002-6300-201004023

• 工艺技术 • 上一篇    下一篇

水解大豆制备食用香精的工艺优化

赵妍嫣,姜绍通,康海超   

  1. 合肥工业大学生物与食品工程学院
  • 收稿日期:2009-03-20 修回日期:2009-09-18 出版日期:2010-02-15 发布日期:2010-12-29
  • 通讯作者: 赵妍嫣 E-mail:zhaoyy33@163.com

Optimization of Millard Reaction for Preparation of Food Flavors from Hydrolyzed Soybean Protein

ZHAO Yan-yan,JIANG Shao-tong,KANG Hai-chao   

  1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
  • Received:2009-03-20 Revised:2009-09-18 Online:2010-02-15 Published:2010-12-29

摘要:

以大豆为原料,通过酸解法制备植物水解蛋白(HVP),以HVP 为原料通过美拉德反应制取肉味香精。通过正交试验获得酸解大豆蛋白的最佳反应条件为水解时间7h、浓硫酸体积分数25%、固液比1:7(g/mL),此时蛋白水解度(DH)达65.26%。在此基础上研究美拉德反应的最佳工艺条件,正交试验结果表明:pH7.0、D- 木糖添加量0.4g/mL、L- 半胱氨酸盐酸盐添加量为0.05g/mL 时,获得的产物香味持久、浓郁。

关键词: 植物水解蛋白(HVP), 香精, 水解, 美拉德反应

Abstract:

To provide a main reactant for the formation of meat flavor in the Millard reaction, soybean was used as the raw material to prepare hydrolyzed vegetable protein (HVP) by acid hydrolysis. The optimal HVP preparation process and Millard reaction were both investigated using orthogonal array design. Results showed that the optimal technological parameters for soybean protein hydrolysis were as follows: sulfuric acid concentration 25% (V/V), solid/liquid ratio 1:7 (g/mL), and hydrolysis time 7 h, and the degree of hydrolysis (DH) obtained using the above hydrolysis process reached up to 65.26%. The optimum Maillard reaction conditions were as follows: pH 7.0, D-xylose amount 0.4 g/mL, and L-cysteine hydrochloride amount 0.05 g/mL.

Key words: hydrolyzed soybean protein, flavor, hydrolysis, Maillard reaction

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