食品科学 ›› 2010, Vol. 31 ›› Issue (3): 60-63.doi: 10.7506/spkx1002-6300-201003013

• 基础研究 • 上一篇    下一篇

不同方法萃取蜂胶挥发油组成及抑菌作用的研究

徐 响1,2,董 捷1,2,丁小宇1,杨佳林1,孙丽萍1,2,*   

  1. 1.中国农业科学院蜜蜂研究所 2.农业部国家蜂产品加工专业分中心
  • 收稿日期:2009-04-01 修回日期:2009-10-21 出版日期:2010-02-01 发布日期:2010-12-29
  • 通讯作者: 徐响 E-mail:xuxiang5000@yahoo.com.cn
  • 基金资助:

    农业部现代农业(蜂)产业技术体系建设专项资金项目(NYCYTX-43);
    中国农业科学院院本级基本科研业务费项目(0032007222)

Propolis Essential Oil Extracted by Different Methods: Chemical Composition Analysis and Antibacterial Activity Evaluation

XU Xiang1,2,DONG Jie1,2,DING Xiao-yu1,YANG Jia-lin1,SUN Li-ping1,2,*   

  1. 1. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China;
    2. National Research Center of Bee Product Processing, Ministry of Agriculture, Beijing 100093, China
  • Received:2009-04-01 Revised:2009-10-21 Online:2010-02-01 Published:2010-12-29
  • Contact: XU Xiang E-mail:xuxiang5000@yahoo.com.cn

摘要:

采用水蒸气蒸馏法和超临界二氧化碳萃取法获得了蜂胶挥发油。GC-MS 分析并鉴定出50 种化合物,两种提取方法所得蜂胶挥发油在成分组成上存在显著差异。抑菌实验表明,两种提取方法获得的挥发油对金黄色葡萄球菌、枯草芽孢杆菌、铜绿假单胞菌具有很强的抑制作用,而超临界法萃取的挥发油的抑菌效果强于水蒸气蒸馏法。

关键词: 超临界二氧化碳萃取法, 水蒸气蒸馏法, 蜂胶, 抑菌作用

Abstract:

In this work, propolis essential oil was obtained by steam distillation extraction and supercritical CO2 extraction, respectively. Added together, 50 components in resulting propolis essential oils were identified by GC-MS method. Propolis essential oils extracted by both methods exhibited strong inhibition effects against Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa in vitro. Stronger inhibition effect of propolis essential oil extracted by supercritical CO2 extraction on the three species of bacteria than that of propolis essential oil extracted by steam distillation extraction was observed.

Key words: supercritical CO2 extraction, steam distillation extraction, propolis, antibacterial activity

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