食品科学 ›› 2009, Vol. 30 ›› Issue (23): 294-296.doi: 10.7506/spkx1002-6300-200923066

• 生物工程 • 上一篇    下一篇

新疆圆柏叶内生菌的分离及其抑菌特性研究

祖丽皮亚·玉努斯   

  1. 新疆大学生命科学与技术学院
  • 收稿日期:2009-02-24 修回日期:2009-08-07 出版日期:2009-12-01 发布日期:2010-12-29
  • 通讯作者: 祖丽皮亚·玉努斯 E-mail:zulfiya_yunus@126.com
  • 基金资助:

    国家自然科学基金项目(30560004);新疆大学青年教师科研启动基金项目(QN040122)

Isolation of Preliminary Study on Endophytic Bacteria in Sabina vulgaris Ant. with Antibacterial Activity

Zulfiya YUNUS   

  1. College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China
  • Received:2009-02-24 Revised:2009-08-07 Online:2009-12-01 Published:2010-12-29
  • Contact: Zulfiya YUNUS E-mail:zulfiya_yunus@126.com

摘要:

对新疆圆柏叶内生菌进行分离,确定分离条件,分离纯化得到7 株菌株,采用打孔法对这7 株菌的发酵液进行抑菌实验。结果表明:其中两株菌的发酵液对枯草芽孢杆菌、金黄色葡萄球菌呈现较强的抑菌活性。通过形态学观察和革兰氏染色,确定1 号菌株为革兰氏阳性球菌,2 号菌株为革兰氏阴性球菌,均无荚膜,无芽孢,不产酸,不产气,淀粉水解为阴性。

关键词: 新疆圆柏, 内生菌, 分离, 抑菌活性

Abstract:

Seven bacteria strains were isolated form leaves of Sabina vulgaris Ant, and antibacterial activity of their fermentation products were evaluated according to the size of their anitbacterial circles. Totally 2 endophytic bacterium exhibited antibacterial activity, which could inhibit the growth of Bacillus subtilis and Staphylococcus aureus. Colonial morphology observation and Gram staining of the 2 endophytic bacterium showed that one bacteria was coccus shaped, gram-positive, and the other was coccus shaped and gram-negative, both without capsule and spore, and without producing acid and amylase.

Key words: Sabina vulgaris Ant, endophytic bacteria, isolation, antibacterial activity

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