食品科学 ›› 2009, Vol. 30 ›› Issue (13): 163-167.doi: 10.7506/spkx1002-6630-200913038

• 生物工程 • 上一篇    下一篇

响应面法对Protamex酶解醇法大豆浓缩蛋白的优化

刘 洁,江连洲* ,张景亮,李 扬   

  1. 东北农业大学食品学院
  • 收稿日期:2008-12-09 修回日期:2009-03-30 出版日期:2009-07-01 发布日期:2010-12-29
  • 通讯作者: 江连洲 E-mail:jlzname@163.com

Optimization of Protamex-catalyzed Hydrolysis of Alcohol-leached Soy Protein Concentrates Using Response Surface Methodology

LIU Jie,JIANG Lian-zhou*,ZHANG Jing-liang,LI Yang   

  1. College of Food, Northeast Agricultural University, Harbin 150030, China
  • Received:2008-12-09 Revised:2009-03-30 Online:2009-07-01 Published:2010-12-29
  • Contact: JIANG Lian-zhou E-mail:jlzname@163.com

摘要:

采用Protamex 蛋白酶对醇法大豆浓缩蛋白进行酶法改性,以氮溶解指数(NSI)为指标,确定酶法改性对提高蛋白溶解度的有效性。在单因素试验基础上采用四因素三水平的响应面分析方法对酶解条件进行优化。确定酶解最佳工艺条件为:pH6.6,温度54℃、底物浓度6%、酶浓度5.5%,此条件下酶解5h,NSI 可达84.45%。

关键词: 响应面分析, 醇法大豆浓缩蛋白, Protamex蛋白酶

Abstract:

Protamex was employed to modify alcohol-leached soy protein concentrate (ALSPC) in this study. With nitrogen solution index (NSI) as the evaluation indicator, a four-factor and three-level response surface analysis was conducted to optimize four hydrolysis parameters including pH, temperature, substrate concentration and enzyme concentration. The results showed that the optimum conditions for enzymatic hydrolysis were as follows: pH 6.6, hydrolysis temperature 54 ℃, substrate concentration 6% and enzyme concentration 5.5%. After the hydrolysis for 5 h under the above conditions, the NSI reached 84.45%.

Key words: response surface methodology, alcohol-leached soy protein concentrate, Protamex

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