食品科学 ›› 2011, Vol. 32 ›› Issue (3 ): 19-22.doi: 10.7506/spkx1002-6630-201103005

• 基础研究 • 上一篇    下一篇

金属离子对木聚糖酶制备阿魏酸糖酯的影响

赵 冰1,2,刘海顺3,王 静1,2,*,张 可4,孙宝国1,2,曹雁平1,2   

  1. 1. 北京工商大学 北京市植物资源研究开发重点实验室 2. 食品添加剂与配料北京高校工程研究中心 3.中储粮北京分公司承德粮油质检中心,河北省承德市双滦区东平台 4.空军航空医学研究所
  • 收稿日期:2010-03-31 修回日期:2011-01-05 出版日期:2011-02-15 发布日期:2011-01-13
  • 通讯作者: 王静 E-mail:jwang810@yahoo.com.cn
  • 基金资助:

    北京市教育委员会面上项目(KM200910011002);北京市科技新星项目(2008B07)

Influence of Ions on Feruloyl Oligosaccharide Preparation from Wheat Bran by Xylanase Hydrolysis

ZHAO Bing1,2,LIU Hai-shun3,WANG Jing1,2,*,ZHANG Ke4,SUN Bao-guo1,2,CAO Yan-ping1,2   

  1. 1. Beijing Key Laboratory of Plant Resource Research and Development, Beijing Technology and Business University, Beijing
    100048, China;2. Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing 100048,
    China;3. Chengde Grain Quality Test Center, China Grain Reserves Corporation Beijing Branch, Shuangluan District, Chengde
    067001, China;4. Air Force Institute of Aviation Medicine, Beijing 100142, China
  • Received:2010-03-31 Revised:2011-01-05 Online:2011-02-15 Published:2011-01-13
  • Contact: WANG Jing1 E-mail:jwang810@yahoo.com.cn

摘要: 采用木聚糖酶制备小麦麸皮中的阿魏酸糖酯。结果表明:添加金属离子会明显影响木聚糖酶的活性,K+、Ca2+、Mn2+、Ag+、Zn2+、Co2+ 对木聚糖酶有激活作用,其中Mn2+、Ag+、Zn2+、Co2+ 有较强的激活作用;而EDTA、Sn2+、Fe2+、Fe3+、Hg2+、Mg2+ 对木聚糖酶有抑制作用,而且高浓度的金属离子也会抑制木聚糖酶的活性。在制备阿魏酸糖酯时添加一定浓度对木聚糖酶有激活作用的金属离子,可以降低木聚糖酶的添加量。

关键词: 麦麸, 金属离子, 木聚糖酶, 阿魏酸糖酯

Abstract: The feruloyl oligosaccharide was prepared from wheat bran by xylanase. The results indicated that the activity of xylanase was strongly affected by metal ions. K+, Ca2+, Mn2+, Ag+, Zn2+, and Co2+ could activate xylanase, and Mn2+, Ag+, Zn2+ and Co2+ showed stronger promoting effect. The activating effect of Zn2+ was positively dependent on its concentration within a certain range, but excessively high concentration inhibited the activity of the enzyme. However, EDTA, Sn2+, Fe2+, Fe3+, Hg2+ and Mg2+ were inhibitors of the enzyme. Less xylanase was required when adding a certain concentration of metal ions during preparation of feruloyl oligosaccharide.

Key words: wheat bran, metal ions, xylanase, feruloyl oligosaccharide

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