食品科学 ›› 2020, Vol. 41 ›› Issue (5): 102-107.doi: 10.7506/spkx1002-6630-20190102-016

• 营养卫生 • 上一篇    下一篇

麦麸中烷基间苯二酚诱导HepG2细胞自噬和凋亡

郭亚洲,杨小明,李月英   

  1. (1.江苏大学食品与生物工程学院,江苏 镇江 212013;2.江苏大学医学院,江苏 镇江 212013)
  • 出版日期:2020-03-15 发布日期:2020-03-23
  • 基金资助:
    国家自然科学基金面上项目(81372404);江苏高校优势学科建设工程资助项目(PAPD)

Alkylresorcinols from Wheat Bran Induced Autophagy and Apoptosis of HepG2 Cells

GUO Yazhou, YANG Xiaoming, LI Yueying   

  1. (1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; 2. School of Medicine, Jiangsu University, Zhenjiang 212013, China)
  • Online:2020-03-15 Published:2020-03-23

摘要: 采用制备色谱从麦麸中制备烷基间苯二酚同系物(alkylresorcinols,ARs),研究其诱导HepG2细胞自噬和凋亡作用。结果表明:经液相色谱-质谱确认制得的烷基间苯二酚为C19:0、C21:0同系混合物;细胞毒性实验结果显示,10~320 μg/mL的ARs能显著抑制HepG2细胞生长(P<0.05、P<0.01、P<0.001),且呈剂量依赖效应;与对照组相比,160 μg/mL ARs作用24 h,HepG2细胞凋亡率极显著升高(P<0.01),剪切型Caspase3、Bax/Bcl-2蛋白表达高度显著升高(P<0.001),Beclin-1、LC3II蛋白表达极显著升高(P<0.01),p62蛋白表达极显著降低(P<0.01)。这些结果表明,ARs可以诱导HepG2细胞自噬,激活Caspase通路诱导HepG2细胞凋亡,抑制HepG2细胞生长。

关键词: 烷基间苯二酚, 麦麸, HepG2细胞, 凋亡, 自噬

Abstract: In this study, two alkylresorcinol homologues C19:0 and C21:0 were isolated by preparative chromatography from wheat bran, and their autophagy and apoptosis-inducing effect on HepG2 cells was studied. The results showed that the structures of alkylresorcinol C19:0 and C21:0 were validated by liquid chromatography-mass spectrometry (LC-MS). Cytotoxicity tests showed that the alkylresorcinols (ARs) at concentrations ranging from 10 to 320 μg/mL could significantly inhibit the growth of HepG2 cells in a dose-dependent manner (P < 0.05, P < 0.01, P < 0.001). Compared with the control group, cell apoptosis rates in the experimental group significantly increased (P < 0.01); the protein expression of cleaved caspase3, Bax/Bcl-2, beclin-1 and LC3II increased but the protein expression of p62 decreased (P < 0.01) after 24 h treatment at 160 μg/mL. Therefore, it was suggested that the alkylresorcinols could induce autophagy in HepG2 cells, and induce apoptosis by activating the caspase-3 pathway, thus inhibiting the growth of HepG2 cells.

Key words: alkylresorcinols, wheat bran, HepG2 cells, apoptosis, autophagy

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