食品科学 ›› 2009, Vol. 30 ›› Issue (23 ): 88-91.doi: 10.7506/spkx1002-6300-200923018

• 基础研究 • 上一篇    下一篇

几种植物提取液的抑真菌特性及复配研究

党 珍,周 涛   

  1. 南京师范大学金陵女子学院食品系
  • 收稿日期:2009-08-09 出版日期:2009-12-01 发布日期:2010-12-29
  • 通讯作者: 党 珍 E-mail:zhoutaoo@sina.com

Antifungal Activity of Several Plant Extracts and Combinatorial Formula

DANG Zhen,ZHOU Tao   

  1. Department of Food Sciences, Ginling College, Nanjing Normal University, Nanjing 210097, China
  • Received:2009-08-09 Online:2009-12-01 Published:2010-12-29
  • Contact: DANG Zhen E-mail:zhoutaoo@sina.com

摘要:

从39 种植物中筛选抑菌作用显著的3 种材料为丁香、肉桂、山奈。用其提取液进行复配实验,确定植物提取液的最佳配比。采用二倍稀释法测定丁香、肉桂、山奈及其复配液对黑曲霉、米曲霉、黑根霉、生香酵母菌的最小抑菌浓度(MIC),并进一步研究复配液对温度、pH 值、紫外光照射的稳定性。结果表明:提取液的最佳配比为5:3:2(V/V)。复配液对供试菌的最小抑菌浓度均为1.25%,与丁香效果大致相同,比肉桂和山奈单独作用有明显增强,并且温度、pH 值、紫外光照射对复配液的稳定性影响不显著。

关键词: 植物提取液, 复配, 最小抑菌浓度(MIC), 稳定性

Abstract:

Clove, cinnamon and kaempferol with remarkable antifungal activity were selected from 39 herbs. In order to obtain an optimal combinatorial formula, minimum inhibitory concentrations (MICs) of three kinds of plant extracts and their various combinatorial formulas to Aspergillus niger, Aspergillus oryzae, Rhizopus nigricans and Saccharomyces were determined through double broth dilution method. Meanwhile, effects of temperature, pH value and UV irradiation on stability of combinatorial formula were also evaluated. The optimal combination ratio of clove, cinnamon and kaempferol extracts was 5:3:2 (V/V). This optimal combinatorial formula exhibited a MIC of 1.25%, which was much lower than those of cinnamon or kaempferia extract. Moreover, temperature, pH and UV irradiation had no effect on stability of combinatorial extracts.

Key words: plant extract, combination, minimum inhibitory concentration (MIC), stability

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