白平菇不同菌株菌丝体的RAPD分析及系统进化关系的研究

食品科学 ›› 2007, Vol. 28 ›› Issue (7): 283-285.

白平菇不同菌株菌丝体的RAPD分析及系统进化关系的研究

宿红艳, 王磊, 刘林德, 蔡德华

鲁东大学生命科学学院；鲁东大学生命科学学院山东烟台264025；山东烟台264025；

出版日期:2007-07-15 发布日期:2011-10-24

RAPD Analysis and Phylogenetic Study of Cultivated Mycelia of Pleurotus ostreatus

SU Hong-Yan, WANG Lei, LIU Lin-De, CAI De-Hua

College of Life Sciences, Lu Dong University, Yantai 264025, China

Online:2007-07-15 Published:2011-10-24

摘要/Abstract

摘要： 本研究采用RAPD技术分析白平菇四个不同株系的DNA序列多态性,用15个随机引物对菌株基因组DNA进行PCR扩增,其中3个引物可以扩增出较好的多态性条带图谱,共产生66条清晰稳定的带。通过对供试菌株遗传相似系数的计算和系统聚类分析,构建了树状聚类图谱,在分子水平上分析了白平菇的种质遗传学差异,为白平菇菌株鉴定提供快速有效的技术和方法。

关键词: 白平菇, RAPD分析, 遗传相似系数, 系统进化关系

Abstract: Four strains of Pleurotus ostreatus were analysised by RAPD (random amplified polymorphic DNA) to investigate the polymorphism of their DNA sequences. The results showed that 3 of 15 arbitray primers could produce 66 obvious and stable DNA bands. The dendrogram was constructed by the similarity coefficient calculation and phylogenetic analysis. This is a rapid and efficient technique to identify the strains of P.floridanus, and analysis their genetic distance on molecular level.

Key words: Pleurotus ostreatus, RAPD analysis, similarity coefficient, phylogenetic relationship

宿红艳, 王磊, 刘林德, 蔡德华. 白平菇不同菌株菌丝体的RAPD分析及系统进化关系的研究[J]. 食品科学, 2007, 28(7): 283-285.

SU Hong-Yan, WANG Lei, LIU Lin-De, CAI De-Hua. RAPD Analysis and Phylogenetic Study of Cultivated Mycelia of Pleurotus ostreatus[J]. FOOD SCIENCE, 2007, 28(7): 283-285.