食品科学 ›› 2005, Vol. 26 ›› Issue (6): 65-68.

• 基础研究 • 上一篇    下一篇

羊肚菌多酚氧化酶性质的研究

 袁江兰, 胡征, 康旭, 万里   

  1. 湖北工业大学生物工程学院
  • 出版日期:2005-06-15 发布日期:2011-09-19

Study on Characteristics of Polyphenoloxidase from Morchella esculenta

 YUAN  Jiang-Lan, HU  Zheng, KANG  Xu, WAN  Li   

  1. Department of Bioengineering, Hubei Polytechnic University
  • Online:2005-06-15 Published:2011-09-19

摘要: 本文研究了温度、pH值等对羊肚菌多酚氧化酶(MEPPO)活力的影响及其热稳定性和以邻苯二酚为底物的动力学。结果表明:MEPPO最适反应温度为50℃;温度低于60℃时,MEPPO的稳定性较高,而在70℃保温4h时,活性几乎完全丧失;MEPPO最适pH值为7.0;以邻苯二酚为底物的最大反应速度Vmax=0.02ΔA/min,Km=0.00588。

关键词: 羊肚菌, 多酚氧化酶, 性质

Abstract: In the paper, characters of polyphenol oxidase from Morchella esculenta were studied, including effect of temperature and pH on activity of PPO, heat stability and reaction kinetics used pyrocatechol as its substrate. The results show that MEPPO is stable relatively below 60℃, the optimum temperature and pH of MEPPO are 50℃ and 7.0 respectively, the maximum velocity of MEPPO reaction V is 0.02 ΔA/min, and the Michaelis constant is 0.00588. max

Key words: Morchella esculenta, polyphenoloxidase, characters