食品科学 ›› 2006, Vol. 27 ›› Issue (10): 288-292.

• 工艺技术 • 上一篇    下一篇

基于径向基神经网络和遗传算法的聚-γ-谷氨酸发酵培养基优化

 周景文,  徐建,  陈守文,  喻子牛   

  1. 华中农业大学农业微生物国家重点实验室
  • 出版日期:2006-10-15 发布日期:2011-11-16

Optimization of Poly-γ- Glutamate Fermentation Medium Based on Radius Basis Function Neural Network and Genetic Algorithm

 ZHOU  Jing-Wen,   Xu-Jian,   Chen-Shou-Wen,   Yu-Zi-Niu   

  1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
  • Online:2006-10-15 Published:2011-11-16

摘要:  为了提高聚-γ-谷氨酸(PGA)的产量,采用正交设计方案对发酵培养基组分中谷氨酸、葡萄糖、柠檬酸、甘油的配比进行试验设计,运用径向基神经网络建立PGA产量与培养基组分浓度之间的预测模型,采用遗传算法对此模型进行全局寻优,得到四种主要组份的最佳配比:谷氨酸21.2g/L、葡萄糖75.4g/L、柠檬酸7.2g/L、甘油10.8g/L,PGA产量达到12.8g/L,采用上述方法优化后的培养基使PGA的产量原始培养基提高了39.1%。

关键词: 聚-&gamma, -谷氨酸, RBF神经网络, 遗传算法, 发酵培养基, 优化

Abstract:  For improving the poly-γ-glutamate (PGA) yield, the orthogonal design was used for the trial design of the formula of medium components: glutamate, glucose, citrate and glycerol, radius basis function neural network (RBFNN) was applied for the predict modeling of the relationships between the PGA yield and the concentration of medium components. Then the genetic algorithm (GA) was used for the global optimization of the model. The optimum combination of the medium was obtained: glutamate 21.2g/L, glucose 75.4g/L, citrate 7.2g/L, glycerol 10.8g/L. The yield of PGA was improved to 12.8g/L, which was increasedby 39.1 % compared to the original medium.

Key words:  , poly-&gamma, -glutamate; RBF neural network; genetic algorithm; fermentation medium; optimization;