食品科学 ›› 2020, Vol. 41 ›› Issue (2): 181-187.doi: 10.7506/spkx1002-6630-20181101-014

• 生物工程 • 上一篇    下一篇

面向规模化应用的竹荪多糖液态深层发酵工艺优化

冯杰,冯娜,刘艳芳,唐庆九,周帅,刘方,张劲松,杨焱   

  1. (农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海 201403)
  • 出版日期:2020-01-25 发布日期:2020-01-19
  • 基金资助:
    2016年度上海市农业领军人才项目;2014年度上海闵行高层次人才科研项目; 上海市农业科学院卓越团队建设计划项目(农科创2017(A-06))

Optimization of Medium Components for Large-Scale Production of Intracellular Polysaccharides from Dictyophora indusiata in Submerged Fermentation

FENG Jie, FENG Na, LIU Yanfang, TANG Qingjiu, ZHOU Shuai, LIU Fang, ZHANG Jingsong, YANG Yan   

  1. (Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China)
  • Online:2020-01-25 Published:2020-01-19

摘要: 利用规模化生产工艺提高竹荪菌丝体胞内多糖得率,通过对竹荪菌丝体液态深层发酵培养基组分的筛选,采用单因素试验与Box-Behnken响应面法确定竹荪菌丝体液态深层发酵的最佳培养基配方为:葡萄糖32.3 g/L,酵母粉4.5 g/L,磷酸二氢钾2.1 g/L,硫酸镁2.5 g/L。在此条件下竹荪胞内多糖得率的预测值为1.23 g/L,摇瓶实验验证胞内多糖得率为1.19 g/L,与预测值相当。在5、30 L和50 L发酵罐中验证胞内多糖得率分别达到1.43、1.28 g/L和1.26 g/L,分别高于预测值16.26%、4.06%和2.43%。本研究优化获得的竹荪菌丝体液态深层发酵工艺有效促进了竹荪胞内多糖的高产,该工艺可为竹荪多糖的规模化生产应用提供较好的参考。

关键词: 竹荪多糖, 菌丝体, 发酵工艺, 优化, 规模化生产

Abstract: The main purpose of this study was to increase the yield of mycelial polysaccharides from Dictyophora indusiata using a large-scale production process. Using a combination of one-factor-at-a-time method and response surface methodology with Box-Behnken design, the optimum medium formulation for the submerged fermentation of D. indusiata was determined as follows: glucose 32.3 g/L, yeast powder 4.5 g/L, potassium dihydrogen phosphate 2.1 g/L, and magnesium sulfate 2.5 g/L. The predicted yield of intracellular polysaccharides was 1.23 g/L, which was close to the experimental value from shake flask fermentation (1.19 g/L). The yields of intracellular polysaccharides in 5-, 30- and 50-L fermentors were 1.43, 1.28 and 1.26 g/L, respectively, which were 16.26%, 4.06% and 2.43% higher than the predicted values, respectively. In this study, the optimized fermentation process gave a high yield of intracellular polysaccharides. Thus, this process provides a good basis for the large-scale production of D. indusiata mycelial polysaccharides.

Key words: Dictyophora indusiata polysaccharide, mycelium, fermentation process, optimization, large-scale production

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