食品科学 ›› 2006, Vol. 27 ›› Issue (10): 326-330.

• 工艺技术 • 上一篇    下一篇

响应面法优化猕猴桃果浆酶解工艺参数研究

 汪志君, 韩永斌, 顾振新, 张丽华   

  1. 扬州大学食品科学与工程学院; 南京农业大学农业部农畜产品加工与质量控制重点实验室;
  • 出版日期:2006-10-15 发布日期:2011-11-16

Optimizing Pectinase Usage in Pretreatment of Kiwifruit Juice for Clarification by Response Surface Methodology

 WANG  Zhi-Jun, HAN  Yong-Bin, GU  Zhen-Xin, ZHANG  Li-Hua   

  1. 1.College of Food Science and Engineering, Yangzhou University, Yangzhou 225001, China; 2.Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2006-10-15 Published:2011-11-16

摘要:  本文研究果胶酶反应温度和作用时间及酶用量对猕猴桃果浆出汁率、果汁澄清度和乙醇不溶物的影响,采用通用旋转设计和响应面分析猕猴桃果浆酶解最优条件。结果表明,猕猴桃果浆的最佳酶解条件为:反应温度为45℃,作用时间为113min,果胶酶用量为0.013%,在此条件下,猕猴桃果浆预测出汁率达到83.30%,乙醇不溶物0.98g/100ml,果汁的澄清度为81.47%。验证试验表明,响应面优化后得到猕猴桃果浆出汁率、果汁澄清度和乙醇不溶物回归方程的相关系数分别为0.958、0.904和0.922。

关键词: 猕猴桃, 果胶酶, 出汁率, 澄清度, 乙醇不溶物

Abstract:  Kiwifruit pulp, treated with pectinase at different duration(40~141min), temperatures(37~54℃) and variousconcentration level of enzyme(0.005%~0.015%), the juice yield, clarity and alcohol insoluble solid were analyzed. And then, the optimum process parameters were determined employing a second-order central composite rotatable design in combination with response surface methodology. The optimum combination was 0.013% pectinase at 45℃ for 113min, the juice yield was 83.30%, the clarity 81.47% and alcohol insoluble solid 0.98g/100ml. Demonstration tests indicated that the R values of juice yield, clarity and alcohol insoluble solid equations were 0.958, 0.904 and 0.922 respectively.

Key words:  , kiwifruit juice; pectinase; yield; clarity; alcohol insoluble solid(AIS);