食品科学 ›› 2006, Vol. 27 ›› Issue (12): 622-626.

• 分析检测 • 上一篇    下一篇

超声波乙醇浸提快速测定苦瓜总皂甙的方法研究

毛清黎,施兆鹏,李玲,刘仲华,朱旗   

  1. 湖南农业大学园艺学院; 华南师范大学生命科学学院;孝感学院生命科学学院;
  • 出版日期:2006-12-15 发布日期:2011-11-23

Study on A Rapid Determination of Total Momordicoside by Ultrasonic Extraction from Bitter Melon(Momordica charantia)

 MAO  Qing-Li, SHI  Zhao-Peng, LI  Ling, LIU  Zhong-Hua, ZHU  Qi   

  1. 1.College of Horticulture, Hunan Agricultural University, Changsha 410128, China; 2.College of Life Science, Xiaogan University, Xiaogan 432100, China; 3.College of Life Science, South China Normal University, Guangzhou 510631, China
  • Online:2006-12-15 Published:2011-11-23

摘要: 本文建立了苦瓜中苦瓜总皂甙超声波乙醇浸提快速测定方法,该法仅需样品0.1g,浸提溶剂80%乙醇2ml,浸提时间仅需30min,不需加热,降低能耗,分析测定效率提高十倍以上。测定结果与常规的乙醇回流没有显著差异。

关键词: 超声波浸提, 快速测定, 苦瓜总皂甙

Abstract: A rapid determination of total momordicoside by ultrasonic extraction from bitter melon (Momordica charantia) was reported in this paper.In the operation of ultrasonic extraction,only 0.1g of sample and 2ml of 80% ethanol were used.Only 30min was necessary for the extraction.As compared with the conventional Soxhlet’s method,the ultrasonic extraction method required shorter extraction time, less solvent reagent and no heating. The determination efficiency of the ultrasonic method was 10 times higher than the Soxhlet,s method.There was no significant difference between the analytical results obtained by those two methods.

Key words: ultrasonic extraction, rapid determination, total momordicoside