食品科学 ›› 2003, Vol. 24 ›› Issue (1): 48-51.

• 基础研究 • 上一篇    下一篇

粘红酵母GLR513生产油脂最佳小型工艺发酵条件的探讨

 施安辉, 周波   

  1. 山东大学生命科学学院微生物学系
  • 出版日期:2003-01-15 发布日期:2011-12-13

Study on Optimum Minitype Fermentation Conditions of Lipid Production by Rhodotorule Glurtinis GLR513

 SHI  An-Hui, ZHOU  Bo   

  • Online:2003-01-15 Published:2011-12-13

摘要: 以GRL513粘红酵母(Rhodtoruleglutinis)的突变株为出发菌株进行小试生产,确定其发酵生产油脂的最佳小型工艺条件为:葡萄糖为碳源,(NH4)2SO4为氮源,碳氮比为70∶1,发酵初始pH5.5,发酵过程回调pH值,接种量为20%,温度为30℃,通过后期补加碳源方式培养72h,最终油脂产量可达菌体干重的67.2%,对该变异菌株产生的油脂进行气相色谱和质谱分析,确定油脂中棕榈油酸含量为33.31%,油酸3.80%,γ-亚油酸0.20%,EPA2.60%,DHA3.60%,总计56.61%。

关键词: 粘红酵母, 发酵条件, 油脂组成与含量

Abstract: Weobtaineda Rhodoruleglutinis mutantGLR 513 byprotoplasts mutagenization(UV.andEMS )tobeput into small scale lipid production .T he best minitype fermentation condi tions as to use glucose as carbon sour ce,(NH 4 ) 2 SO 4 as nitrogen source,C /Nas 70∶1,starting pHas 5.5,inoculative amount as20%and temperature as30℃.The yield of lipid production co uld reach 67.2%of dry thallius weigh t by replenishing carbon source in anaphase for 72h .We confirmed the co mponent of lipid by gas -liquid chrom atogrphy and mass spectrum: 2003,Vol.24,No.1palmitoleic acid 33.31%,oleic acid3.80%,γ-linoleic acid 0.20%,EPA2.60%and DHA3.60%with the amount as 56.61%.

Key words: Rhodotoruleglutinis, fermentation condition, componen t and constant of lipid