食品科学 ›› 2000, Vol. 21 ›› Issue (5): 49-53.

• 分析检测 • 上一篇    下一篇

酱油中黄酮含量测定方法的优化

 张海德, 张水华, 肖凯军, 李琳, 郭祀远   

  1. 华南理工大学食品与生物工程学院
  • 出版日期:2000-05-15 发布日期:2011-11-30

Optimization Study of Flavonoids Determination in Soya Sauce

 ZHANG  Hai-De, ZHANG  Shui-Hua, XIAO  Kai-Jun, LI  Lin, GUO  Si-Yuan   

  • Online:2000-05-15 Published:2011-11-30

摘要: 对酱油中总黄酮的Davis法测定影响因素作了较深入研究,并优化分析过程,获得较佳操作条件。即采用pH=12的碱水浸提30min,然后样液在pH=6下测定。该法的标准偏差在3.87%范围内。平均回收率为99.16%。通过对两种广州酱油的测定,证明优化全分析方法的精密度和准确度好。

关键词: 酱油, 黄酮化合物, 含量

Abstract: In this paper the determination conditions of flavones in soya sauve by Davis method were optimized.Flavonoids were extracted by dilute alkaline solution at pH12 for 30adn.Then the pH of thes solution was adjusted to 6 by citric acid solution.The content was measured by a colorimeter method.The precision (stdevp%) of this method was less than 3. 87%, and the average recovery was 99 .16% .The total content of flavonoids in two kinds of soy sauce made in Guangzhou was in the range of 45~50mg/100g.

Key words: Soy sauce Flavonoids Content