氨气敏电极法定量测定大豆脲酶活性

食品科学 ›› 1993, Vol. 14 ›› Issue (9): 64-68.

氨气敏电极法定量测定大豆脲酶活性

徐茂军, 乐培恩, 郑凯

淮海工学院食品与化学工程系

出版日期:1993-09-15 发布日期:2011-12-30

Quantitative Determination of Urease in Soybean by Ammonia Gas Sensing Electrode

Xu-Mao-Jun, LE Pei-恩, ZHENG Kai

Online:1993-09-15 Published:2011-12-30

摘要/Abstract

摘要： 以氨气敏电极测定单位时间内脲酶分解尿素所生成的氨数量定量表示大豆脲酶活性高低,并对大豆脲酸的最适反应条件及氨气敏电极的测试条件进行了研究。结果表明,大豆脲酶的最适温度为50℃;最适PH为 8．0;适宜的底物浓度为 0．02 mol/L八尿素,氨气敏电极稳态响应的线性范围为 10-14101mol/LNH4CL.样品测定的回收率在97．5%103．3%之间,相对标准偏差在0．55%2．65%之间。

关键词: 氨气敏电极, 大豆脲酶

Abstract: Urease in soybean is determined quantitatively by ammonia gas sensing electrode based on the ammonia produced from the urease hydrolysis reaction of urea. The effects of temperature, pH and urea concentration on characteristic of urease in soybean is studied. The optimum temperature of soybean urease is 50℃. The pH value at which soybean urease shows supreme hydrolysis reaction is 8. 0. The suitable urea concentration is 0. 02 mol/ L. The linear range of steady-state response of ammonia gas sensing probe is 10 4-10-1 mol/L NH4Cl. This method has been applied to analysis of urease in soybean and gives recoveries of 97. 5% -103. 3% and standard deviation of 0. 55%-2. 65%.

Key words: , Ammonia Gas Sensing Electrode Urease；

徐茂军, 乐培恩, 郑凯. 氨气敏电极法定量测定大豆脲酶活性[J]. 食品科学, 1993, 14(9): 64-68.

Xu-Mao-Jun, LE Pei-恩, ZHENG Kai. Quantitative Determination of Urease in Soybean by Ammonia Gas Sensing Electrode[J]. FOOD SCIENCE, 1993, 14(9): 64-68.

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