Abstract:

In order to extract the high-purity sesamin, this paper researches isolation and purification of sesamin from sesame seed meal by means of macro-porous resin. Results indicated that AB-8 resin and HP-20 resin have much higher static adsorption rate and desorption rate for sesamin, which adsorption rates are respectively 9.24mg/g and 9.28mg/g, and the desorption rates are respectively 94.81% and 94.35%. By carrying on the dynamic adsorption and the desorption experiment with AB-8, the result indicates that the higher the sample concentration, the bigger the adsorption, and the less injection velocity is more advantageous to the adsorption. It’s a much better effect by using 95% ethyl alcohol desorption, and reasonable amount of desorption liquid is 10 times the volume of the column (BV), and if the elution speed is smaller, the higher content sesamin can be obtained by desorption. Taking everything into consideration, it is more appropriate to select injection velocity and elution velocity for 1.0ml/min. The purity of sesamin is from crude extract of 12.88% up to 70.56% by macro-porous resin purification.