高效液相色谱法检测三两半药酒中阿魏酸

食品科学 ›› 2012, Vol. 33 ›› Issue (20): 176-179.

高效液相色谱法检测三两半药酒中阿魏酸

张星海，周晓红

浙江经贸职业技术学院应用工程系

收稿日期:2011-07-21 修回日期:2012-07-27 出版日期:2012-10-25 发布日期:2012-11-09
通讯作者: 张星海 E-mail:zjuxy2001@163.com

Determination of Ferulic Acid in Sanliangban Medicinal Liquor by HPLC

Received:2011-07-21 Revised:2012-07-27 Online:2012-10-25 Published:2012-11-09
Contact: Zhang Xing-Hai E-mail:zjuxy2001@163.com

摘要/Abstract

摘要： 目的：建立一种高效液相色谱法测定三两半药酒中阿魏酸含量的方法。方法：采用高效液相色谱，波长316nm、柱温25℃、流速1.0mL/min、进样量20µL；从不同品牌色谱柱、流动相类型及组成比例3个方面筛选阿魏酸检测条件；从线性范围、回收率、精密度、重现性及稳定性等方面进行考察。结果：以Eclipse XDB-C18(250mm×4.60mm，5µm)为色谱柱，乙腈-0.1%磷酸(15:85，V/V)为流动相检测效果良好。该方法在1.15～114.70μg/mL内线性关系良好(R2=0.9999)，进样精密度(RSD)为0.4%(n=6)，进样重现性RSD为1.2%(n=6×2)，平均回收率为99.5%，RSD为0.5%(n=6×2)，在18h内检测8次，RSD为1.4%。结论：本法简便、准确、专属性强，重现性好，可用于该药酒质量控制。

关键词: 阿魏酸, 三两半药酒, 高效液相色谱法, 质量分析

Abstract: Objective: To develop an HPLC method for determining ferulic acid in Sanliangban medicinal liquor. Methods: At a flow rate of 1.0 mL/min, the prepared analyte was injected at a level of 20 μL for detection at 316 nm. The column temperature was 25 ℃. Efforts were made to determine optimal column type and mobile phase composition. Moreover, the developed HPLC method was investigated in terms of its linear range, recovery rate, precision, reproducibility and stability. Results: Eclipse XDB-C18 column (250 mm × 4.60 mm, 5 μm) was the best column for separation of ferulic acid. Good separation was achieved using a mobile phase made up of acetonitrile and 0.1% phosphoric acid (15:85, V/V). The calibration curve for ferulic acid was linear within the range of 1.15–114.70 μg/mL (R2 = 0.9999). The precision (RSD), reproducibility (RSD), recovery rate and stability (RSD) of this method were 0.4% (n = 6), 1.2% (n = 6 × 2), 99.5% and 0.5% (n = 6 × 2), respectively. The RSD for 8 repeated determinations within 18 h was 1.4%. Conclusion: This method is simple, accurate, specific, reproducible and applicable for quality control of Sanliangban medicinal liquor.

Key words: ferulic acid, Sanliangban medicinal liquor, HPLC, quality analysis

中图分类号:

G927.2

张星海，周晓红. 高效液相色谱法检测三两半药酒中阿魏酸[J]. 食品科学, 2012, 33(20): 176-179.

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