泥鳅蛋白酶解工艺条件的优化

doi:10.7506/spkx1002-6630-201307040

食品科学 ›› 2013, Vol. 34 ›› Issue (7): 190-195.doi: 10.7506/spkx1002-6630-201307040

泥鳅蛋白酶解工艺条件的优化

李莹1，曾晓雄2，周剑忠1，黄开红1，胡冰2

1.江苏省农业科学院农产品加工研究所，江苏南京 210014；2.南京农业大学食品科技学院，江苏南京 210095

收稿日期:2012-02-06 修回日期:2013-01-31 出版日期:2013-04-15 发布日期:2013-03-20
通讯作者: 李莹 E-mail:hijoly@163.com
基金资助:
江苏省科技支撑项目

Enzymatic Preparation of Antihypertensive Peptides from Loach (Misgurnus anguillicaudatus) Protein

LI Ying1，ZENG Xiao-xiong2，ZHOU Jian-zhong1，HUANG Kai-hong1，HU Bing2

1. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China； 2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received:2012-02-06 Revised:2013-01-31 Online:2013-04-15 Published:2013-03-20
Contact: Ying LI E-mail:hijoly@163.com

摘要/Abstract

摘要： 以泥鳅蛋白为原料，酶解制备具有降血压活性的短肽。从9种蛋白酶中筛选出菠萝蛋白酶的酶解液具有较高降压活性，IC50值为0.65mg/mL。在单因素试验的基础上，采用和Box-Behnken和响应面法(RSM)优化了酶解泥鳅蛋白的工艺条件，以ACE抑制率为指标，探讨酶与底物的比值([E]/[S])、酶解温度和酶解时间对ACE抑制率的影响。结果表明：制备降血压肽的最佳酶解条件为[E]/[S]4.9‰、酶解时间5.9h、酶解温度54.4℃、pH6.5、底物质量分数30%，该条件下制备的泥鳅蛋白酶解产物的ACE抑制率为88.92%。

关键词: 泥鳅, 酶解, 降血压肽, ACE抑制活性

Abstract: The aim of this study was to prepare antihypertensive peptides by enzymatic hydrolysis of loach protein. Bromelain was found to be the best protease for the preparation of peptides with high antihypertensive activity (IC50 = 0.65 mg/L) among nine proteases. Based on one-factor-at-a-time experiments, Box-Behnken experimental design coupled with response surface methodology (RSM) was employed to optimize the hydrolysis process of loach protein. The ACE inhibitory rate of antihypertensive peptides was investigated with respect to three hydrolysis parameters including enzyme/substrate ratio, temperature and time. The optimum hydrolysis conditions for preparing antihypertensive peptides were determined as 4.9‰, 6.5, 30%, 54.4 ℃ and 5.9 h for enzyme/substrate ratio, pH, substrate concentration, hydrolysis temperature and hydrolysis time, respectively. Under these conditions, the ACE inhibitory rate of loach protein hdyrolysate was 88.92%.

Key words: loach (Misgurnus anguillicaudatus), enzyme hydrolysis, antihypertensive peptide, ACE inhibitory activity

中图分类号:

Q556.3
TS218

李莹1，曾晓雄2，周剑忠1，黄开红1，胡冰2. 泥鳅蛋白酶解工艺条件的优化[J]. 食品科学, 2013, 34(7): 190-195.

LI Ying1，ZENG Xiao-xiong2，ZHOU Jian-zhong1，HUANG Kai-hong1，HU Bing2. Enzymatic Preparation of Antihypertensive Peptides from Loach (Misgurnus anguillicaudatus) Protein[J]. FOOD SCIENCE, 2013, 34(7): 190-195.

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泥鳅蛋白酶解工艺条件的优化

Enzymatic Preparation of Antihypertensive Peptides from Loach (Misgurnus anguillicaudatus) Protein

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