响应面法优化短短芽孢杆菌FM4B的发酵培养基

食品科学 ›› 2012, Vol. 33 ›› Issue (15): 257-261.

响应面法优化短短芽孢杆菌FM4B的发酵培养基

芮广虎，胡雪芹*，殷坤，张洪斌

合肥工业大学医学工程学院

收稿日期:2011-06-17 修回日期:2012-06-25 出版日期:2012-08-15 发布日期:2012-09-07
通讯作者: 胡雪芹 E-mail:hxq2468@hotmail.com
基金资助:
合肥工业大学大学生创新性实验计划项目

Medium Optimization for Antifungal Substance Production by Brevibacillus brevis FM4B Using Response Surface Methodology

Received:2011-06-17 Revised:2012-06-25 Online:2012-08-15 Published:2012-09-07
Contact: Xue-Qin HU E-mail:hxq2468@hotmail.com

摘要/Abstract

摘要： 采用Plackett-Burman法考察发酵培养基中各组分对抗真菌活性物质产量的影响。结果表明：葡萄糖、蔗糖、K2HPO4的质量浓度对抗真菌活性物质产量影响显著。再采用最陡爬坡路径逼近最大响应区域，并结合Box-Behnken响应面法对3个主要因素进行分析，得到优化的发酵培养基组成(g/L)：葡萄糖6.1、蔗糖31.3、蛋白胨23.1、K2HPO4 0.825、MgSO4·7H2O 0.5。采用该法优化所得的培养基，其抑菌圈面积增加了42%。

关键词: 短短芽孢杆菌, 生物防治, Box-Behnken试验, 响应曲面法, 培养基优化

Abstract: Plackett-Burman design was used to evaluate the effects of 9 medium components on the production of antifungal substance by Brevibacillus brevis FM4B. The results showed that glucose, peptone and K2HPO4 were the most important medium components. Steepest ascent procedure and response surface methodology based on Box-Behnken experimental design were employed to optimize the three medium components. The optimal fermentation medium for producing antifungal substance was composed of glucose 6.1 g/L, peptone 23.1 g/L, sucrose 31.3 g/L, K2HPO4 0.825 g/L and MgSO4·7H2O 0.5 g/L. The inhibition spot area of the optimized culture medium after fermentation with Brevibacillus brevis FM4B was increased by 42% compared to that of the original medium.

Key words: Brevibacillus brevis, biological control, central composite design, response surface methodology, medium optimization

中图分类号:

中图分类号：S432.4

芮广虎，胡雪芹*，殷坤，张洪斌. 响应面法优化短短芽孢杆菌FM4B的发酵培养基[J]. 食品科学, 2012, 33(15): 257-261.

参考文献

[1] 沈世红. 要重视我国的农药污染问题[J]. 生物学教学, 2002, 27(3):37-38.
[2] 贺思彤. 对我国食品安全法律制度的思考[C]. 中国环境科学学会学术年会优秀论文集, 2008, 1701-1704.
[3] 郝晓娟, 刘波, 葛慈斌, 等. 短短芽孢杆菌JK-2菌株抑菌活性物质产生条件的优化[J]. 河北农业科学, 2009, 13(6):46-49.
[4] 戴晓燕, 关桂兰. 两株对辣椒疫霉菌有拮抗作用的拮抗菌分泌蛋白的研究[J]. 中国生物防治, 1999, 15(2):81-84.
[5] 翟茹环, 尚玉珂, 刘峰, 等. 枯草芽孢杆菌G8抗菌蛋白的理化性质和抑菌作用[J]. 植物保护学报, 2007, 34(6):592-596.
[6] 陈曼, 李赤, 邱逸斯, 等. 富贵竹黑腐病拮抗菌H5的抑菌机制及相关特性研究[J]. 微生物学通报, 2008, 35(4):529-532.
[7] 彭清忠，张惟材，朱厚础. 枯草芽孢杆菌表达系统的研究进展[J]. 生物技术通讯, 2001, 12(3):220-225.
[8] 胡雪芹，芮广虎，周雪梅，等. 生防菌FM4B的鉴定及抗菌物质的性质研究[J].上海交通大学学报(农业科学版), 2010, 29(1):75-80.
[9] CHEN Xiaochun, BAI Jianxin, CAO Jiaming,et al.Medium optimization for the production of cyclic adenosine 3’,5’-monophsphate by Microbacterium sp.no.205 using response surface methodology[J]. Bioresource Technology, 2009, 100:919-924.
[10] 周丹，刘世玲，边银丙. 响应面法优化富硒金针菇菌株发酵培养基的研究[J].食用菌学报, 2006, 13(1):33-36.
[11] 王普，孙立明，何军邀. 响应面法优化热带假丝酵母104菌株产羰基还原酶发酵培养基[J]. 生物工程学报, 2009, 25(6)：863-868.
[12] P.Ambati,C.Ayyanna. Optimizing medium constituents and fermentation conditions for ciric acid production from palmyra jiggery using response surface method[J]. World Journal of Microbiology and Biotechnology, 2001, 17:331-335.
[13] 冯培勇, 赵彦宏, 张丽. 响应面法优化黑曲酶产纤维素酶发酵条件[J]. 食品科学, 2009 , 30(23):335-339.
[14] B.V.V. Ratnam,M.Narasimha Rao,M.Damodar Rao,et al.Optimization of fermentation condition for the production of ethanol from sago starch using response surface methodogy[J]. World Journal of Microbiology and Biotechnology, 2003, 19:523-526.
[15] B.V.V. Ratnam, S. Subba Rao, M.Damodar Rao,et al. Optimization of medium constiuents and fermentation conditions for the production of ethanol form palmyra jiggery using response methodology[J]. World Journal of Microbiology and Biotechnology, 2005, 21:399-404.
[16] Veera VRB,Subba RS,Damodara RM,et al.Optimization of fermentation condition for the production of ethanol from sago starch by co-immobilized amyloglucosidase and cells of Zymomonas mobilis using response surface methodology[J]. Enzyme and Microbial Technology, 2006, 38:209-214.
[17] JIANG Longfa. Optimization of fermentation conditions for pullulan production by Aureobasidium pullulan using response surface methodology[J]. Carbohydrate Polymers, 2010, 79:414-417
[18] THOMPSON DD. Response surface experimentation[J]. Food Processing and Preservation, 1982, 6(3):155-188.
[19] 毋锐琴, 杜双奎, 李志西, 等. 细菌纤维素发酵培养基的优化及超微观结构分析[J]. 生物工程学报, 2008, 24(6):1068-1074.
[20] 欧宏宇, 贾仕儒. SAS软件在微生物培养条件中的应用[J]. 天津轻工业学院, 2001, 1:14-18.