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• 工艺技术 •    下一篇

壳聚糖固定化果胶酶对玛咖汁澄清效果的研究

徐涓1,孙彦琳2,张弘1,周梅村3,郑华1,张雯雯1   

  1. 1. 中国林业科学研究院资源昆虫研究所
    2. 昆明理工大学
    3. 昆明理工大学化学工程学院
  • 收稿日期:2013-03-01 修回日期:2013-07-19 出版日期:2013-08-25 发布日期:2013-09-03
  • 通讯作者: 张弘 E-mail:kmzhhong@163.com
  • 基金资助:
    国家林业公益性行业科研专项

Effects of Pectinase Immobilized by Chitosan on Clarification of Maca Juice

  • Received:2013-03-01 Revised:2013-07-19 Online:2013-08-25 Published:2013-09-03
  • Contact: Zhang Hong E-mail:kmzhhong@163.com

摘要: 在玛咖汁贮存过程中,果胶、水溶性蛋白质和多酚易导致其混浊,需进行澄清处理,为此,采用壳聚糖固定化果胶酶澄清玛咖汁,以达到快捷、经济的效果。本文在单因素试验的基础上,采用响应面分析法(Response surface methodology,RSM)模拟出壳聚糖固定化果胶酶用量、澄清时间和澄清温度对玛咖汁澄清度影响的二次多项式回归数学模型。结果表明:壳聚糖固定化果胶酶用量43.50g/L、澄清时间104min、澄清温度50℃,玛咖汁的澄清度为95.78%。玛咖汁澄清后,易引起果汁混浊的果胶、水溶性蛋白质、总酚的沉降率依次为60.73%、32.93%和9.06%,玛咖汁中主要活性成分芥子油苷、水溶性生物碱、VC和总糖的损失率依次为6.92%、3.35%、3.25%和1.82%,表明在响应曲面法优化条件下,壳聚糖固定化果胶酶澄清能较好的保留玛咖汁中的的主要活性成分。在其最优条件下,壳聚糖固定化果胶酶连续使用10次后,玛咖汁的澄清度仍能达到75.49%,说明该固定化酶具有一定的操作稳定性。

关键词: 玛咖汁, 壳聚糖固定化果胶酶, 澄清

Abstract: Precipitation was easy to occur during the storage of maca juice, so clarification was necessary. In order to achieve fast and economic effect, immobilized pectinase clarification was used. Response surface methodology (RSM) was employed to construct a quadratic regression model describing the effects of addition quantity of immobilized pectinase, time and temperature on clarification of maca juice. The optimization results showed that the optimum conditions were 43.50g/L of dosage and 50℃ of temperature load for 104min, the luminousness of maca juice was 95.78%. Before and after clarification of the maca juice, the loss rate of pectin, water-soluble proteins and total phenols which would affect the stability of juice were 60.73%, 32.93% and 9.06%. The loss rate of glucosinolates, water soluble alkaloids, VC and total sugar were 6.92%, 3.35%, 3.25% and 1.82%. It showed that the nutrients could be well preserved by immobilized pectinase clarification. The transmittance of immobilized pectinase was 75.49% after repeated ten times. It showed that the immobilized pectinase was stable.

Key words: Maca juice, Pectinase immobilized by chitosan, Clarification