UPLC-Q-TOF-MS检测槲皮素代谢产物在大鼠血浆及组织中的分布

摘要/Abstract

摘要： 目的：采用四极杆-飞行时间串联质谱(Q-TOF-MS)检测分析大鼠口服槲皮素单体后血浆和组织中的代谢产物。方法：血浆和组织样品采用Sephadex LH-20固相萃取小柱进行分离纯化，使用四极杆-飞行时间串联质谱仪检测，以体积分数0.1%甲酸溶液-乙腈为流动相梯度洗脱，电喷雾离子源负离子条件下进行扫描，鉴定大鼠给药槲皮素单体后血浆及组织中的代谢产物。结果：槲皮素在血液中主要以其葡糖醛酸、硫酸酯结合物的形式存在，约占全部代谢物的73.70%，一部分也以其脂肪酸结合物的形式存在，约占26.30%；此外，槲皮素在体内分布广泛，在组织中的含量由高到低依次为乳腺＞子宫＞卵巢＞肾上腺＞心＞脾＞肝＞肾。结论：Q-TOF-MS可快速、有效地鉴定出SD大鼠血浆及组织中的槲皮素代谢产物，为今后进行槲皮素与人类健康效应相关的研究提供参考。

关键词: 槲皮素, 四极杆-飞行时间串联质谱仪, 葡糖醛酸, 硫酸酯, 代谢物

Abstract: Objective: To determine quercetin metabolites in plasma and tissues of SD rats after intragastric administration of quercetin aglycone by quadrupole-time of flight-mass spectrometry (Q-TOF-MS). Methods: The pre-processed samples of plasma and tissues were separated and purified by Sephadex LH-20 solid-phase column. The collected aliquots were analyzed by Q-TOF-MS in negative mode based on gradient elution with a mobile phase composed of 0.1% formic acid (A) and acetonitrile (B) to identify the metabolites of quercetin. Results: Q-TOF-MS analysis showed that quercetin was primarily metabolized to glucuronides and sulfates (73.70%) as well as esters (26.30%). Moreover, quercetin and quercetin metabolites were widely distributed in rat tissues, especially in the female reproductive organs in SD rats. Its concentration in tissues was as follows: breast > uterus > ovary > adrenal gland > heart > spleen > liver > kidney. Conclusions: Q-TOF-MS can quickly and effectively identify quercetin metabolites in SD rats’ plasma and tissues and provide reference to study the relationship between quercetin or its metabolites and health effect in the future.

Key words: quercetin, quadrupole-time of flight-mass spectrometry, glucuronides, sulfates, metabolites

中图分类号:

邢萌萌朱科学聂少平李娜李文娟杨希谢明勇. UPLC-Q-TOF-MS检测槲皮素代谢产物在大鼠血浆及组织中的分布[J]. 食品科学, 0, (): 0-0.

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