食品科学

• 工艺技术 •    下一篇

香蕉花黄酮的提取工艺优化及其抑制α-糖苷酶活性研究

王 会,盛占武,金志强,马蔚红,施瑞城   

  1. 1.海南大学食品学院,海南 海口 570228;2.中国热带农业科学院海口实验站,海南 海口 570102
  • 出版日期:2013-08-25 发布日期:2013-09-03

Optimization of Extraction of Flavonoids from the Banana Flower and Its α-Glucosidase Inhibitory Activity

WANG Hui,SHENG Zhan-wu,JIN Zhi-qiang,MA Wei-hong,SHI Rui-cheng   

  1. 1. College of Food Science and Technology, Hainan University, Haikou 570228, China;
    2. Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou 570102, China
  • Online:2013-08-25 Published:2013-09-03

摘要:

以芦丁为标样,香蕉花中黄酮提取量为参考指标,探讨乙醇回流提取香蕉花黄酮工艺,并用α-葡萄糖苷酶对香蕉花乙醇提取物进行活性分析,确定α-葡萄糖苷酶抑制类型。结果表明:用乙醇回流提取香蕉花黄酮的最佳工艺条件为乙醇溶液体积分数60%、料液比1:30(g/mL)、提取温度65℃、提取时间1h,平均黄酮提取量为26.25mg/mL;α-葡萄糖苷酶对香蕉花乙醇提取物有较强的酶抑制活性,抑制率可达到88.56%;α-葡萄糖苷酶抑制剂的类型是竞争性抑制,α-葡萄糖苷酶Km=674.074μg/mL。

关键词: 香蕉花, 黄酮, 工艺优化, &alpha, -糖苷酶抑制活性

Abstract:

This study aimed to optimize the ethanol reflux extraction of flavonoids from banana flower to obtain maximum
extraction efficiency and evaluate the α-glucosidase inhibitory activity and mechanism of the resulting ethanol extract. The
optimal extraction conditions that provided maximum extraction efficiency (26.25 mg/mL on average) were found to be 60%
ethanol as the extraction solvent with a solid-to-solvent ratio of 1:30 (g/mL) and temperature 65 ℃ for an extraction duration
of 1 h. The ethanol extract obtained under the optimized conditions had potent α-glucosidase inhibitory activity providing an
inhibition rate of 88.56%. The inhibition mechanism was competitive and the Km 674.074 μg/mL.

Key words: banana flower;flavonoids;process optimization;&alpha, -glucosidase inhibitory activity