LAMP法检测转基因大豆A2704-12品系

doi:10.7506/spkx1002-6630-201324042

食品科学

LAMP法检测转基因大豆A2704-12品系

邵碧英，陈文炳，曾莹，陈彬，郑晶，缪婷玉，彭娟

1.福建出入境检验检疫局，福建福州 350001；2.福建农林大学食品科学学院，福建福州 350002

出版日期:2013-12-25 发布日期:2014-01-03

Detection of Genetically Modified Soybean Line A2704-12 in Soybean and Its Products by LAMP Method

SHAO Bi-ying，CHEN Wen-bing，ZENG Ying，CHEN Bin，ZHENG Jing，MIAO Ting-yu，PENG Juan

1. Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China；
2. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Online:2013-12-25 Published:2014-01-03

摘要/Abstract

摘要：

根据转基因大豆A2704-12品系的特异序列设计了4套环介导等温扩增(LAMP)引物。通过反应温度优化、特异性测定，筛选出1套特异性强的LAMP引物。对筛选到的LAMP引物进行反应条件、反应体系的优化，建立了转基因大豆A2704-12品系的LAMP检测方法。结果表明：建立的LAMP检测方法能特异、稳定地检测转基因大豆A2704-12品系，检测限达到0.1%(m/m)。应用建立的LAMP方法检测大豆及其制品中转基因大豆A2704-12品系，检测结果与实时荧光聚合酶链式反应(PCR)结果完全一致。

关键词: 环介导等温扩增, 转基因大豆A2704-12品系, 特异性检测

Abstract:

According to the specific sequence of genetically modified soybean line A2704-12, four sets of primers for loopmediated
isothermal amplification (LAMP) were designed. One set of highly specific primers was screened through optimizing
the reaction temperature and determining the specificity. An LAMP method for the detection of genetically modified soybean line
A2704-12 was developed after optimizing the reaction conditions and system. The specificity, sensitivity and stability of the LAMP
method were evaluated. The results showed that the LAMP method could detect specifically and stably transgenic soybean A2704-12
strain with a low detection limit of 0.1%. The results of detection the A2704-12 strain in soybean and its products by the LAMP
method were same as those from the real-time fluorescent PCR method.

Key words: loop-mediated isothermal amplification (LAMP), genetically modified soybean line A2704-12, specific detection

邵碧英，陈文炳，曾莹，陈彬，郑晶，缪婷玉，彭娟. LAMP法检测转基因大豆A2704-12品系[J]. 食品科学, doi: 10.7506/spkx1002-6630-201324042.

SHAO Bi-ying，CHEN Wen-bing，ZENG Ying，CHEN Bin，ZHENG Jing，MIAO Ting-yu，PENG Juan. Detection of Genetically Modified Soybean Line A2704-12 in Soybean and Its Products by LAMP Method[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201324042.

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LAMP法检测转基因大豆A2704-12品系

Detection of Genetically Modified Soybean Line A2704-12 in Soybean and Its Products by LAMP Method

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