关键词: 鼠源性成分；肉类掺假；环介导等温扩增技术；线粒体基因；羊肉制品

Abstract: Loop-mediated isothermal amplification (LAMP) was employed for the qualitative detection of murine-derived ingredients in mutton products. Specific primers targeting the murine mitochondrial genes (rat COX gene KP244683.1 and mouse 16S rRNA gene KY018919.1) were designed, and the reaction conditions were optimized to determine the optimal reaction system. The specificity, sensitivity, and stability were evaluated using simulated mixed samples. LAMP was used to detect commercially available mutton products, and the obtained results were compared with those obtained with real-time PCR. The results showed that the method established in this study had good specificity and stability. The limits of detection (LODs) for rat- and mouse-derived ingredients were 0.1% and 0.5%, respectively. The results of LAMP were highly consistent with those of real-time fluorescent PCR. Therefore, the LAMP method proved simple, efficient, and accurate, which could provide a new choice for rapid diagnosis of murine meat adulteration and primary diagnosis, and could be used as a new rapid detection method for murine-derived ingredients in mutton products.

Key words: murine-derived components; meat adulteration; loop-mediated isothermal amplification; mitochondrial genes; mutton products