葡萄籽原花青素的提取及其抗紫外线活性测定

doi:10.7506/spkx1002-6630-201418013

食品科学

葡萄籽原花青素的提取及其抗紫外线活性测定

杨健1，袁春龙1,2,*，任亚梅3，方玉飞1，杨晓雁1，张世杰1

1.西北农林科技大学葡萄酒学院，陕西杨凌 712100；2.陕西省葡萄与葡萄酒工程技术研究中心，陕西杨凌 712100；
3.西北农林科技大学食品科学与工程学院，陕西杨凌 712100

出版日期:2014-09-25 发布日期:2014-09-17
通讯作者: 袁春龙
基金资助:
西北农林科技大学科技创新重点项目（ZD2013017）

Extraction and Anti-Ultraviolet Activity of Proanthocyanidins from Grape Seed

YANG Jian1 , YUAN Chun-long1,2,*, REN Ya-mei3, FANG Yu-fei1, YANG Xiao-yan1, ZHANG Shi-jie1

1. College of Enology, Northwest A & F University, Yangling 712100, China;
2. Shaanxi Engineering Research Center for Viti-Viniclture, Yangling 712100, China;
3. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China

Online:2014-09-25 Published:2014-09-17
Contact: YUAN Chun-long

摘要/Abstract

摘要：

采用快速溶剂萃取法提取葡萄籽脱脂粉中的原花青素，通过单因素试验和正交试验优化提取工艺，最终获得葡萄籽原花青素提取物（grape seed proanthocyanidins extracts，GSPE）干粉。快速简便地测定不同质量浓度的GSPE乙醇溶液的防晒系数以反映抗紫外线活性，并探究GSPE乙醇溶液质量浓度对其抗紫外线活性的影响。结果表明，快速溶剂萃取法以乙醇做安全提取剂，最佳提取参数为萃取压强1 100 psi、萃取时间14 min、萃取温度70 ℃、乙醇体积分数70%，在此条件下，提取原花青素得率为71.62 mg/g，纯度为41.53%；1 000 μg/mL的原花青素提取物乙醇溶液的防晒系数值达24.755 6±0.759 9，具有显著抗紫外线活性；GSPE抗紫外线活性与质量浓度在一定范围内呈显著正相关。

关键词: 抗紫外线, 葡萄籽原花青素提取物, 紫外分光光度计法, 防晒系数, 快速溶剂萃取法

Abstract:

An accelerated solvent extractor (ASE) was used to extract proanthocyanidins from defatted grape seed powder.
Grape seed proanthocyanidin extracts (GSPE) were obtained under the optimal operating conditions established by one
factor-at-a-time and orthogonal array design methods. We evaluated the anti-ultraviolet (UV) activity of GSPE at various
concentrations in ethanol by determining sun-protection factor (SPF) by a rapid and simple method with the aim of clarifying
the concentration-effect relationship. The optimal extraction conditions were found to be 14 min of extraction at 70 ℃with 70%
ethanol/water mixture (V/V) at a pressure of 1 100 psi. Under these conditions, the yield and purity of GSPE were 71.62 mg/g
and 41.53%, respectively. The SPF of 1 000 μg/mL proanthocyanidins in ethanol was 24.755 6 ± 0.759 9, which
confirmed the anti-UV activity of GSPE. A significant positive concentration-effect relationship was found within a certain
concentration range.

Key words: anti-UV activity, grape seed proanthocyanidin extracts (GSPE), ultraviolet spectrophotometry method, sunprotection factor (SPF), accelerated solvent extractor (ASE)

中图分类号:

TS201.2

杨健1，袁春龙1,2,*，任亚梅3，方玉飞1，杨晓雁1，张世杰1. 葡萄籽原花青素的提取及其抗紫外线活性测定[J]. 食品科学, doi: 10.7506/spkx1002-6630-201418013.

YANG Jian1,YUAN Chun-long1,2,*, REN Ya-mei3, FANG Yu-fei1, YANG Xiao-yan1, ZHANG Shi-jie1. Extraction and Anti-Ultraviolet Activity of Proanthocyanidins from Grape Seed[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201418013.

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葡萄籽原花青素的提取及其抗紫外线活性测定

Extraction and Anti-Ultraviolet Activity of Proanthocyanidins from Grape Seed

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