关键词: HEK293, 氧化应激, 顺铂, 葡萄籽原花青素提取物, 肾毒性

Abstract: Objective: To investigate the protective effect of grape seed proanthocyanidin extract (GSPE) against cisplatin (CDDP)-induced nephrotoxicity in human embryo kidney (HEK) 293 cells and its possible mechanisms. Methods: The effects of GSPE and CDDP on the in vitro growth of HEK 293 cells and the effect of GSPE on CDDP-induced nephrotoxicity were observed by MTT assay. TBARS assay was used to evaluate the effect of GSPE on GSPE-induced lipid peroxidation. The effect of GSPE on CDDP-induced glutathione depletion was examined by colorimetrically determining absorbance resulting from the reaction between DTNB and hydroxyl compounds. Low concentrations of GSPE could remarkably enhance the activity of HEK 293 cells. CDDP-induced nephrotoxicity in these cells was obviously inhibited by appropriate concentrations of GSPE. Additionally, GSPE could also alleviate CDDP-induced glutathione depletion. Conclusion: GSPE has the potential to inhibit CDDP-induced nephrotoxicity in HEK 293 cells, and the possible mechanism is associated with the strong antioxidant function and free radical quenching activity of GSPE.

Key words: cisplatin, GSPE, nephrotoxicity, HEK293, oxidative stress