新疆酸李提取物对脂多糖诱导RAW264.7炎症模型细胞氧化应激损伤的缓解作用及机制

doi:10.7506/spkx1002-6630-20250424-199

食品科学 ›› 2025, Vol. 46 ›› Issue (20): 223-232.doi: 10.7506/spkx1002-6630-20250424-199

新疆酸李提取物对脂多糖诱导RAW264.7炎症模型细胞氧化应激损伤的缓解作用及机制

萨拉麦提·艾迪热斯，阿依加·马里克，古丽菲热·伊力哈木，艾再提·克里木，阿塔吾拉·铁木尔，尼格尔热依·亚迪卡尔

（1.新疆维吾尔自治区农业科学院农产品加工研究所，新疆农产品加工与保鲜重点实验室，新疆乌鲁木齐 830091；2.新疆农业大学食品科学与药学学院，新疆乌鲁木齐 830052）

出版日期:2025-10-25 发布日期:2025-09-17
基金资助:
新疆维吾尔自治区“天池英才”引进计划——青年博士计划（第一批）项目；新疆农业科学院农业科技创新稳定支持专项（xjnkywdzc-2023003-2-7；xjnkywdzc-2024003-38；xjnkywdzc-2025001-65）

Alleviative Effect and Mechanism of Prunus cerasifera Ehrhart Extract on Oxidative Stress Injury in Lipopolysaccharide-Stimulated RAW264.7 Cells

Salamaiti AIDIRESI, Ayijia MALIKE, Gulifeire YILIHAMU, Aizaiti KELIMU, Atawula TIEMUER, Nigeerreyi YADIKAER

(1. Xinjiang Key Laboratory of Agricultural Products Processing and Preservation, Institute of Agricultural Processing, Xinjiang Academy of Agricultural Sciences, ürümqi 830091, China;2. College of Food Science and Pharmacy, Xinjiang Agricultural University, ürümqi 830052, China)

Online:2025-10-25 Published:2025-09-17

摘要/Abstract

摘要： 本研究探讨新疆酸李（Prunus cerasifera Ehrhart）提取物对脂多糖（lipopolysaccharide，LPS）诱导RAW264.7炎症模型细胞的抗炎作用及其潜在的分子机制。首先，对新疆酸李提取物进行乙醇梯度洗脱，并分别检测不同体积分数乙醇溶液洗脱部位的多酚和黄酮含量以及抗氧化活性；其次，针对体积分数70%乙醇溶液洗脱组分（PCEE-70），分析其对LPS诱导的RAW264.7炎症细胞中抗氧化及氧化应激有关生化指标的影响；最后，通过蛋白免疫印迹（Western blot，WB）实验检测核因子κB（nuclear factor kappa B，NF-κB）p65和磷脂酰肌醇3-激酶（phosphoinositide 3-kinase，PI3K）/蛋白激酶B（protein kinase B，AKT）/c-Jun氨基末端激酶（c-Jun N-terminal kinase，JNK）信号通路有关磷酸化及非磷酸化蛋白的表达水平。结果表明，PCEE-70相较于其他洗脱组分具有较高的多酚和黄酮含量，并具有较强的多种抗氧化活性。PCEE-70能明显降低LPS诱导的RAW264.7炎症细胞中NO、活性氧和丙二醛含量，提高乳酸脱氢酶、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活力，降低白细胞介素（interleukin，IL）-6、IL-1β和肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）促炎因子的水平。此外，WB结果表明，PCEE-70能明显降低LPS诱导的RAW264.7炎症细胞中TNF-α、p-NF-κB p65、p-NF-κB抑制蛋白、p-PI3K、p-AKT及p-JNK蛋白的表达。综上，PCEE-70能抑制LPS诱导RAW264.7炎症细胞炎性因子的分泌，从而延缓氧化应激反应对细胞的损伤，其作用机制与抑制NF-κB和PI3K/AKT信号通路的激活有关。

关键词: 新疆酸李提取物；RAW264.7细胞；抗炎及氧化应激；核因子κB信号通路；磷脂酰肌醇3-激酶/蛋白激酶B信号通路

Abstract: This study aimed to investigate the anti-inflammatory effects of Prunus cerasifera Ehrhart extract (PCEE) on lipopolysaccharide (LPS)-stimulated RAW264.7 cells and to elucidate its underlying molecular mechanism. First, PCEE was fractionated by gradient elution with ethanol, and the separated fractions were evaluated for total polyphenol and flavonoid contents, as well as antioxidant activity. Next, the 70% ethanol-eluted fraction (PCEE-70) was selected to measure its effects on biochemical indexes related to antioxidant defense and oxidative stress in LPS-stimulated RAW264.7 cells. Finally, Western blot (WB) was employed to detect the expression levels of phosphorylated and non-phosphorylated proteins associated with the nuclear factor kappa B (NF-κB) p65 and phosphoinositide 3-kinase/protein kinase B/c-Jun N-terminal kinase (PI3K/AKT/JNK) signaling pathways. The experimental results demonstrated that PCEE-70 had higher contents of polyphenols and flavonoids along with stronger antioxidant activities compared to the other fractions. PCEE-70 significantly inhibited the levels of nitric oxide (NO), reactive oxygen species (ROS) and malondialdehyde (MDA), while enhancing the activities of lactate dehydrogenase (LDH), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). It also reduced the levels of the pro-inflammatory cytokines interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α). Furthermore, WB results indicated that PCEE-70 significantly downregulated the expression of TNF-α, p-NF-κB p65, p-IκB, p-PI3K, p-AKT, and p-JNK proteins. In conclusion, PCEE-70 can inhibit the secretion of inflammatory factors in LPS-induced RAW264.7 cells, thereby mitigating oxidative stress-induced cellular damage. Its action mechanism is associated with the suppression of NF-κB and PI3K/AKT signaling pathway activation.

Key words: Prunus cerasifera Ehrhart extract; RAW264.7 cells; anti-inflammation and oxidative stress; nuclear factor kappa B signaling pathway; phosphoinositide 3-kinase/protein kinase B/c-Jun N-terminal kinase signaling pathways

中图分类号:

萨拉麦提·艾迪热斯，阿依加·马里克，古丽菲热·伊力哈木，艾再提·克里木，阿塔吾拉·铁木尔，尼格尔热依·亚迪卡尔. 新疆酸李提取物对脂多糖诱导RAW264.7炎症模型细胞氧化应激损伤的缓解作用及机制[J]. 食品科学, 2025, 46(20): 223-232.

Salamaiti AIDIRESI, Ayijia MALIKE, Gulifeire YILIHAMU, Aizaiti KELIMU, Atawula TIEMUER, Nigeerreyi YADIKAER. Alleviative Effect and Mechanism of Prunus cerasifera Ehrhart Extract on Oxidative Stress Injury in Lipopolysaccharide-Stimulated RAW264.7 Cells[J]. FOOD SCIENCE, 2025, 46(20): 223-232.

[1]	陈晨郭家棋孔松芝谢海生梁美茵陈锦蕙利紫芮. 鳕鱼皮多肽抗小鼠慢性酒精性肝、脑损伤作用[J]. 食品科学, 0, (): 0-0.
[2]	田琴陈晓鹭于福来. 草豆蔻果挥发油代谢组及抑菌活性时空特异性分析[J]. 食品科学, 0, (): 0-0.
[3]	周堂王吉乔温皓林芷淇朱红波常月胡炜彦张荣平陈兴龙. 樟叶越桔的酚类成分：MS/MS裂解行为和神经保护作用[J]. 食品科学, 0, (): 0-0.
[4]	刘舒鹏黄厚钰王笑雪郝巨辉李卫东. 基于超高效液相色谱-四级杆-静电场轨道阱联用-质谱技术与网络药理学揭示金银花与忍冬叶化学成分差异及抗衰老机制[J]. 食品科学, 0, (): 0-0.
[5]	刘舒鹏, 黄厚钰, 王笑雪, 郝巨辉, 李卫东. 基于超高效液相色谱-四极杆-静电场轨道阱质谱技术与网络药理学揭示金银花与忍冬叶化学成分差异及抗衰老机制[J]. 食品科学, 2024, 45(20): 1-11.
[6]	刘慧茹李文玉陈立国王昊周芳韩利文. 基于高分辨质谱及斑马鱼模型研究磷虾油的磷脂组成特征及降血糖活性[J]. 食品科学, 0, (): 0-0.
[7]	胡璇黄英王凯王丹谢小丽赵建平陈晓鹭于福来. 益智精油化学成分分析及其对单增李斯特菌的抑菌机理[J]. 食品科学, 0, (): 0-0.
[8]	胡璇，黄英，王凯，王丹，谢小丽，赵建平，陈晓鹭，于福来. 益智精油化学成分分析及其对单核细胞增生李斯特氏菌的抑菌机理[J]. 食品科学, 2024, 45(16): 140-150.
[9]	刘兴龙丁传波佘鑫欣陈雪艳赵迎春姜颖 sadia 刘文丛郑毅男. 黑参多糖抗疲劳作用的分子机制[J]. 食品科学, 0, (): 0-0.
[10]	江国勇，雷艾彤，杨莹，余强，谢建华，陈奕. 黑灵芝多糖对丙烯酰胺致大鼠肝脏氧化损伤的保护作用[J]. 食品科学, 2020, 41(1): 121-126.
[11]	郭时印，李林，周虹，唐忠海，苏小军，李清明. 1-脱氧野尻霉素对α-葡萄糖苷酶的抑制作用机制[J]. 食品科学, 2019, 40(5): 45-50.
[12]	张伟，张勇，尹震花，康文艺. 超声辅助离子液体微萃取-反相液相色谱法测定人工蛹虫草活性成分[J]. 食品科学, 2017, 38(8): 179-183.
[13]	张鹏赵辉李士明骆焱平. 槌果藤乙纯提取物保护酿酒酵母免受氧化损伤机制研究[J]. 食品科学, 0, (): 0-0.
[14]	曹纯洁陈美珍叶天文. 末水坛紫菜多糖除蛋白工艺及其抗氧化活性研究[J]. 食品科学, 0, (): 0-0.
[15]	谢飞,曹纯洁,陈美珍*,叶天文. 响应面试验优化末水坛紫菜多糖除蛋白工艺及其抗氧化活性[J]. 食品科学, 2016, 37(22): 77-84.

新疆酸李提取物对脂多糖诱导RAW264.7炎症模型细胞氧化应激损伤的缓解作用及机制

Alleviative Effect and Mechanism of Prunus cerasifera Ehrhart Extract on Oxidative Stress Injury in Lipopolysaccharide-Stimulated RAW264.7 Cells

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价