巢湖蓝藻酸性多糖的理化性质及其体外抗氧化作用

doi:10.7506/spkx1002-6630-201505002

摘要/Abstract

摘要：

目的：以从巢湖蓝藻中分离纯化的一种多糖（acidic polysaccharide from Cyanobacteria of Chaohu，CHAP）为研究对象，对其纯度、分子质量、光谱特征、单糖组成、抗氧化作用进行研究，旨在为水华蓝藻资源进行综合开发利用提供有价值的参考。方法：采用60 ℃的热水抽提蓝藻，采用硫酸铵去除其中蛋白质，粗多糖经DEAE-52阴离子交换层析柱分离并用Sephadex G-150进行纯化得到纯化多糖CHAP。结果：CHAP中多糖和蛋白质含量分别为91.49%和0.18%，紫外-可见光谱表明CHAP不含核酸，高效液相色谱（high performance liquid chromatography，HPLC）测定CHAP的分子质量为2.209×105 D，离子色谱（ion chromatography，IC）表明CHAP由6.36%的阿拉伯糖、35.47%的葡萄糖、5.03%的木糖、17.09%的半乳糖、27.36%的甘露糖及一种未知单糖构成。红外光谱表明CHAP是具有α-D-半乳吡喃糖的特征吸收峰的吡喃糖，CHAP的抗氧化作用表明：CHAP对1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基和超氧阴离子自由基（O2－·）有较强的清除能力，其IC50分别为276 μg/mL和186.05 μg/mL。CHAP对羟自由基（·OH）的清除能力较弱，清除率不到10%。

关键词: 巢湖蓝藻, 酸性多糖, 理化性质, 抗氧化作用

Abstract:

Objective: To the study physico-chemical characteristics and antioxidant activities of acidic polysaccharide
extracted from Cyanobacteria from the Chaohu Lake. Methods: Acidic polysaccharide was extracted by hot water (60 ℃)
from Cyanobacteria. After deproteinization by ammonium sulfate method, the neutral and acidic polysaccharides were
pooled from the elutes of DEAE-52 gel column. Then, the acidic polysaccharides were further loaded onto Sephadex G-150
column and a purified acidic polysaccharide, CHAP, was obtained. HPLC, UV, IR, and IC were used for the analysis of
CHAP for purity, molecular weight, monosaccharide composition, spectroscopic characteristics and antioxidant activity.
Results: The CHAP obtained contained 91.49% polysaccharides and 0.18% proteins without the presence of nucleic
acids. The mean molecular weight of CHAP was 2.209 × 105 D. The monosaccharide composition of CHAP included
L-arabinose (6.36%), glucose (35.47%), D (+) xylose (5.03%), D-mannose (27.36%), D-galactose (17.09%), and unknown
monosaccharides. Infrared spectrum showed that CHAP was mainly composed of pyranose-polysaccharides and had a
characteristic absorption peak of α-D-galactopyranosyl. The results of antioxidant activity indicated that CHAP had a strong
ability to scavenge both superoxide anion free radicals and DPPH free radicals, with IC50 values of 186.05 and 276 μg/mL,
respectively. However, almost no scavenging effect against hydroxyl free radical was observed.

Key words: Cyanobacteria from the Chaohu Lake, acidic polysaccharide, physico-chemical characteristics, antioxidant activity

中图分类号:

TS244.5

李志平1，张弛2，周维清2，纪启芳2，孔小卫2，李玉成1,*. 巢湖蓝藻酸性多糖的理化性质及其体外抗氧化作用[J]. 食品科学, doi: 10.7506/spkx1002-6630-201505002.

LI Zhiping1, ZHANG Chi2, ZHOU Weiqing2, JI Qifang2, KONG Xiaowei2, LI Yucheng1,*. Physico-chemical Characteristics and Antioxidant Activity of Acidic Polysaccharide Extracted from Cyanobacteria from Chaohu Lake[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201505002.

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巢湖蓝藻酸性多糖的理化性质及其体外抗氧化作用

Physico-chemical Characteristics and Antioxidant Activity of Acidic Polysaccharide Extracted from Cyanobacteria from Chaohu Lake

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