EGCG对抗肿瘤所致心肌损伤的保护作用

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EGCG对抗肿瘤所致心肌损伤的保护作用

宁佳鸣¹,张妍淞²,姚于飞³,王喆¹,李露¹,陈家俊¹,李文娟²

1. 南昌大学
2. 南昌大学食品科学与技术国家重点实验室
3. 中国人民解放军第九四医院呼吸科

收稿日期:2015-07-21 修回日期:2016-04-27 出版日期:2016-06-15 发布日期:2016-06-27
通讯作者: 李文娟 E-mail:243832875@qq.com
基金资助:
国家自然（青年）科学基金;江西省科技厅自然科学基金;江西省教育厅自然科学基金

The protective effect of EGCG on myocardial injury by antitumor

¹, ¹, ¹, ¹, ¹, ¹,

Received:2015-07-21 Revised:2016-04-27 Online:2016-06-15 Published:2016-06-27

摘要/Abstract

摘要： 摘要：目的：观察表没食子儿茶素没食子酸酯（EGCG）的抗肿瘤作用，研究EGCG对抗肿瘤药物阿霉素（ADR）所致小鼠心肌损伤的保护作用及其机制。方法：建立S-180小鼠实体瘤模型，随机分为4组：对照组、ADR组、EGCG组、EGCG+ADR组。分离肿瘤与心肌组织，分别测定组织内乳酸脱氢酶（LDH）、肌酸激酶（CK)和锰超氧化物歧化酶（Mn-SOD）的活性；流式细胞仪检测细胞凋亡、活性氧（ROS）及线粒体膜电位（△Ψm）。结果：与对照组相比，EGCG和ADR能显著增加肿瘤细胞凋亡作用，且ADR+EGCG作用更强。与ADR组相比，EGCG+ADR可显著降低心肌组织中ADR引起的LDH和CK的活性增加。同时，EGCG+ADR中可显著增加心肌组织的抗氧化酶MnSOD活性，减少ROS的生成，增加线粒体膜电位。结论：EGCG具有抗肿瘤作用，且与ADR合用可显著增强ADR抗肿瘤作用。同时可通过提高线粒体的抗氧化防御，削弱氧化应激，稳定线粒体膜电位，对ADR所致心肌损伤发挥保护作用。

关键词: 关键词：肿瘤, 阿霉素, 活性氧, 儿茶素

Abstract: Abstract: Objective: Based on observing the effect of Epigallocatechin gallate (EGCG) on antitumor, we will futher study the protective effect of EGCG on myocardial injury triggered by adriamycin (ADR) as an anti-tumor drug and the mechanism. Methods: A S-180 tumor-bearing mice model was established and randomly divided into four groups: Control group, ADR group, EGCG group and EGCG+ADR group. After separating tumor tissue and myocardial tissue, lactate dehydrogenase (LDH), creatine kinase (CK) and Mn-superoxide dismutase (MnSOD) activities were measured with the instruction of kits. And apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (△Ψm) level were measured by flow cytometry. Results: Compared with Control group, EGCG and ADR could significantly increase the apoptosis of tumor cell. And the combination of EGCG and ADR had more significantly effect. Compared with ADR group, EGCG+ADR could significantly reduce LDH and CK activities in myocardial tissue triggered by ADR. At the same time, the combination of EGCG and ADR could significantly increase Mn-SOD activity in myocardial tissue, reduce the production of ROS and keep the balance of △Ψm. Conclusions: EGCG itself had anti-tumor effect, and could enhance the effect with ADR. In addition, EGCG had protective effect on myocardial damage caused by ADR by improving mitochondrial antioxidant defense capacity, impairing oxidative stress and balancing △Ψm.

Key words: Key words: tumor, adriamycin, reactive oxygen species, EGCG

中图分类号:

Q946.84+1

宁佳鸣张妍淞姚于飞王喆李露陈家俊李文娟. EGCG对抗肿瘤所致心肌损伤的保护作用[J]. 食品科学, 0, (): 0-0.

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EGCG对抗肿瘤所致心肌损伤的保护作用

The protective effect of EGCG on myocardial injury by antitumor

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