食品科学 ›› 2023, Vol. 44 ›› Issue (4): 64-70.doi: 10.7506/spkx1002-6630-20220421-266

• 食品化学 • 上一篇    下一篇

罗望子种仁球蛋白-EGCG共价复合物的制备及其在乳化香肠中的应用

杨杨,王梦桔,王悦,边鑫,范婧,马春敏,石彦国,李笑梅,张娜   

  1. (哈尔滨商业大学食品工程学院,黑龙江 哈尔滨 150028)
  • 发布日期:2023-03-01
  • 基金资助:
    “十四五”国家重点研发计划重点专项(2021YFD2100902-3);国家自然科学基金面上项目(32072258); 黑龙江省青年科技人才托举项目(2022QNTJ010);哈尔滨商业大学博士科研项目(22BQ21)

Preparation of Tamarind Seed Globulin-EGCG Covalent Complex and Its Application in Emulsified Sausages

YANG Yang, WANG Mengju, WANG Yue, BIAN Xin, FAN Jing, MA Chunmin, SHI Yanguo, LI Xiaomei, ZHANG Na   

  1. (College of Food Engineering, Harbin University of Commerce, Harbin 150028, China)
  • Published:2023-03-01

摘要: 通过碱处理法制备罗望子种仁球蛋白(tamarind seed globulin,TSG)-表没食子儿茶素没食子酸酯((-)-epigallocatechin-3-gallate,EGCG)共价化合物,采用紫外-可见光谱分析TSG-EGCG共价复合物结合率并优化结合条件。采用色差计、质构分析仪和扫描电子显微镜研究TSG-EGCG共价复合物添加量对乳化香肠蒸煮损失、保水性和质构的影响。结果表明:当TSG与EGCG质量比1∶0.1、反应pH 9.5、反应温度30 ℃时,复合物的结合率达到最大值。与添加TSG的乳化香肠相比,添加TSG-EGCG复合物的乳化香肠微观结构更加紧密,立体凝胶网络更加均匀清晰,同时蒸煮损失率、保水性及质构特性得到明显改善。综上,TSG-EGCG复合物可以改良乳化香肠的感官品质,且当添加量为2%时,乳化香肠的品质得到明显改善。

关键词: 罗望子种仁球蛋白;表没食子儿茶素没食子酸酯;共价复合;乳化香肠;品质

Abstract: In this study, a covalent complex between tamarind seed globulin (TSG) and epigallocatechin-3-gallate (EGCG) was prepared by alkali treatment. UV-visible spectroscopy was used to analyze the binding rate of TSG-EGCG covalent complex and the binding conditions were optimized. The effects of adding different amounts of TSG-EGCG covalent complex on the cooking loss, water-holding capacity and texture of emulsified sausages were studied using a color difference meter, a texture analyzer and a scanning electron microscope (SEM). The results showed that reaction at pH 9.5 and 30 ℃ with a mass ratio of TSG to EGCG of 1:0.1 gave the maximum binding rate. Compared with TSG, the microstructure of emulsified sausages added with TSG-EGCG complex was more compact, the three-dimensional gel network was more uniform and clear, and the cooking loss, water-holding capacity and texture properties were significantly improved. In conclusion, TSG-EGCG complex can improve the sensory quality of emulsified sausages, and the quality of emulsified sausages can be significantly improved by adding 2% of TSG-EGCG complex.

Key words: tamarind seed globulin; epigallocatechin gallate; covalent complex; emulsified sausage; quality

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