罗非鱼鱼皮胶原多肽对体外肠道肿瘤的影响

doi:10.7506/spkx1002-6630-201521037

食品科学

罗非鱼鱼皮胶原多肽对体外肠道肿瘤的影响

江虹锐1,2，邵勇2，刘小玲2，白洋3，米顺利4，杨莉1

1.广西医科大学公共卫生学院，广西南宁 530021；2.广西大学轻工与食品工程学院，广西南宁 530004；
3.广东百维生物科技有限公司，广东化州 525100；4.百洋水产集团股份有限公司，广西南宁 530007

出版日期:2015-11-15 发布日期:2015-12-03

Effects of Collagen Peptides from Tilapia Skin on Colon Carcinoma in Vitro

JIANG Hongrui1,2, SHAO Yong2, LIU Xiaoling2, BAI Yang3, MI Shunli4, YANG Li1

1. Public Health of Guangxi Medicine University, Nanning 530021, China; 2. College of Light Industry and Food Engineering,
Guangxi University, Nanning 530004, China; 3. Guangdong Baiwei Biological Technology Co. Ltd., Huazhou 525100, China;
4. Baiyang Aquatic Group, Inc., Nanning 530007, China

Online:2015-11-15 Published:2015-12-03

摘要/Abstract

摘要：

目的：体外研究罗非鱼鱼皮胶原多肽对人结肠癌细胞系LoVo增殖能力的影响，并初步探讨其作用机理。方法：体外培养人结肠癌LoVo细胞，噻唑蓝（methylthiazolyldiphenyl-tertrazolium bromide，MTT）法检测罗非鱼鱼皮胶原多肽对细胞增殖的影响；荧光探针染色法观察细胞膜及核的变化；激光共聚焦显微镜观察细胞内活性氧（reactive oxygen species，ROS）表达；比色法检测细胞线粒体呼吸链酶复合物Ⅰ和Ⅲ的活性。结果：罗非鱼鱼皮胶原多肽对LoVo细胞的增殖具有一定的抑制作用，当质量浓度为100 mg/mL、作用48 h时，抑制率达到55.03%（P＜0.05）；与空白对照组相比，经胶原多肽作用后，LoVo细胞核浓缩，细胞膜出现不完整，细胞凋亡；且细胞内活性氧水平表达显著增加（P＜0.05），细胞内线粒体呼吸链复合物Ⅲ的活性显著降低（P＜0.05），但对线粒体呼吸链复合物Ⅰ的活性无影响。结论：罗非鱼鱼皮胶原多肽对人结肠癌细胞具有一定的增殖抑制作用，可能与抑制线粒体呼吸链复合物Ⅲ活性，使胞内ROS水平增加，破坏细胞膜形态有关。

关键词: 罗非鱼鱼皮, 胶原多肽, 肠道肿瘤, 活性氧, 线粒体呼吸链复合物

Abstract:

Objective: To investigate the effects of collagen peptides from tilapia skin on the proliferation ability of humancolon carcinoma cell line LoVo in vitro and its possible mechanism. Methods: The inhibitory effects of collagen peptidesfrom tilapia on the proliferation of LoVo cells were observed by MTT assay. Fluorescent staining method was used toanalyze the effect on cell membrane and nucleus. Laser confocal microscopy was used to examine reactive oxygen species(ROS) expression in LoVo cells. The activities of mitochondrial respiration chain complex I and III were analyzed bycolorimetry. Results: Collagen peptides from tilapia skin showed certain inhibitory effect on the proliferation of LoVocells. After being treated by 100 mg/mL of collagen peptides for 48 h, the growth inhibitory effect rate reached 55.03%(P < 0.05). Compared with the control group, the nucleus of LoVo cells was shrunk and cell membrane was destructed afterbeing treated by collagen peptides. Meanwhile, the ROS level was significantly increased (P < 0.05). Collagen peptidessignificantly inhibited the activity of complex III. Conclusion: Collagen peptides from tilapia skin have inhibitory effects onthe proliferation of human colon carcinoma cells, which is probably associated with the inhibited activity of mitochondrialrespiration chain complex III in LoVo cells, promoting ROS expression and destructing the cell membrane in LoVo cells.

Key words: tilapia skin, collagen peptides, colon carcinoma, reactive oxygen species (ROS), mitochondrial respiratory chain complex

中图分类号:

TS254.6

江虹锐，邵勇，刘小玲，白洋，米顺利，杨莉. 罗非鱼鱼皮胶原多肽对体外肠道肿瘤的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201521037.

JIANG Hongrui, SHAO Yong, LIU Xiaoling, BAI Yang, MI Shunli, YANG Li. Effects of Collagen Peptides from Tilapia Skin on Colon Carcinoma in Vitro[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201521037.

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罗非鱼鱼皮胶原多肽对体外肠道肿瘤的影响

Effects of Collagen Peptides from Tilapia Skin on Colon Carcinoma in Vitro

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