近冰温贮藏对杏果实冷害及活性氧代谢的影响

doi:10.7506/spkx1002-6630-20190426-350

食品科学 ›› 2020, Vol. 41 ›› Issue (7): 177-183.doi: 10.7506/spkx1002-6630-20190426-350

近冰温贮藏对杏果实冷害及活性氧代谢的影响

李亚玲，崔宽波，石玲，祝兆帅，李玲，刘严，朱璇

（1.新疆农业大学食品科学与药学学院，新疆乌鲁木齐 830052；2.新疆农业科学院农业机械化研究所，新疆乌鲁木齐 830091）

出版日期:2020-04-15 发布日期:2020-04-20
基金资助:
国家自然科学基金地区科学基金项目（31860462）；自治区公益性科研院所项目（KY2019029）

Effect of Near Freezing Temperature Storage on Chilling Injury and Active Oxygen Metabolism of Apricot Fruit

LI Yaling, CUI Kuanbo, SHI Ling, ZHU Zhaoshuai, LI Ling, LIU Yan, ZHU Xuan

(1. College of Food Science and Pharmacy, Xinjiang Agricultural University, ürümqi 830052, China; 2. Agricultural Mechanization Institute, Xinjiang Academy of Agricultural Sciences, ürümqi 830091, China)

Online:2020-04-15 Published:2020-04-20

摘要/Abstract

摘要： 为研究近冰温贮藏对杏果实冷害及活性氧代谢的影响，本实验以新疆库车小白杏作为实验材料，分别将其贮藏于近冰温（－1.5～－1.0 ℃）和冷藏（4～6、1～2 ℃）条件下49 d，每7 d取样，测定杏果实的冷害发病率，冷害指数和过氧化氢酶（catalase，CAT）、超氧化物歧化酶（superoxide dismutase，SOD）、过氧化物酶（peroxidase，POD）活力，超氧阴离子自由基（O2－·）产生速率，过氧化氢（hydrogen peroxide，H2O2）、丙二醛（malondialdehyde，MDA）含量及细胞膜透性。结果表明：与普通冷藏（4～6、1～2 ℃）相比，近冰温贮藏可有效推迟冷害发病时间并降低冷害发病率，显著提高杏果实SOD的活力（P＜0.05），延缓冷藏期间POD、CAT活力的下降，抑制H2O2含量的升高和O2－·的产生，减少了MDA的积累和细胞膜透性的增加。因此，近冰温贮藏可提高杏果实的抗冷性和贮藏性。

关键词: 杏果实, 近冰温贮藏, 冷害, 活性氧代谢

Abstract: The purpose of this work was to investigate the effect of near freezing temperature (NFT) storage on chilling injury and active oxygen metabolism of apricot fruit. ‘Xiaobaixing’ apricots from Kuche, Xinjiang were stored separately at near freezing temperature (?1 to ?1.5 ℃) and low temperature (4–6 or 1–2 ℃) for 49 days. Chilling injury incidence, chilling injury index, the activities of catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD), superoxide anion radical (O2-·) production rate and the contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA), as well as cell membrane permeability were measured at every seven days. The results showed that compared with low temperature storage, NFT storage effectively delayed the onset of chilling injury, reduced the incidence of chilling injury, significantly increased the activity of SOD (P < 0.05), delayed the decline of POD and CAT activity, inhibited the content of H2O2 and O2-· generation rate, retarded the increase in MDA content and membrane permeability. Therefore, NFT storage could improve the cold resistance and storability of apricot fruit.

Key words: apricot fruit, near freezing temperature storage, chilling injury, active oxygen metabolism

中图分类号:

TS255.36

李亚玲，崔宽波，石玲，祝兆帅，李玲，刘严，朱璇. 近冰温贮藏对杏果实冷害及活性氧代谢的影响[J]. 食品科学, 2020, 41(7): 177-183.

LI Yaling, CUI Kuanbo, SHI Ling, ZHU Zhaoshuai, LI Ling, LIU Yan, ZHU Xuan. Effect of Near Freezing Temperature Storage on Chilling Injury and Active Oxygen Metabolism of Apricot Fruit[J]. FOOD SCIENCE, 2020, 41(7): 177-183.

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近冰温贮藏对杏果实冷害及活性氧代谢的影响

Effect of Near Freezing Temperature Storage on Chilling Injury and Active Oxygen Metabolism of Apricot Fruit

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