关键词: 降亚硝酸盐, 乳酸菌, 驯化, 抑菌, 鉴定

Abstract: Objective Screening the lactic acid bacteria strains that can efficiently degrade the nitrite,and establish theoretical basis for the development of functional dairy products and environmental protection.Method Isolated and purified 13 lactic acid bacterias from the samples such as sauerkraut, cheese , tested the ability of the target strains degrades nitrite by the method of hydrochloric acid naphthalene ethylenediamine. Using biochemistry and 16 s rDNA method to identified the highest nitrite-degrading lactic acid bacteria . Result Obtained a biodegradable nitrite strain JS3 , the strain nitrite degradation rate was 83.39%, through domestication compound screening and cultivate conditions optimization, the degradation rate increased to 93.47%, the strain can also inhibit the growth of G+ and G- bacteria. Strain was identified to be Lactobacillus zeae, named L.zeae. JS3.Conclusion The strain JS3 have efficient ability of nitrite degradation, it can be used as future good strains of probiotic preparations.

Key words: degrading nitrite, lactic acid bacteria, domestication, antibacterial, identification