食品科学 ›› 2024, Vol. 45 ›› Issue (6): 55-63.doi: 10.7506/spkx1002-6630-20230613-098

• 生物工程 • 上一篇    下一篇

基于RBL-2H3细胞脱颗粒抑制作用的体外抗过敏活性益生菌的筛选及鉴定

马丁,秦双霞,郝慧超,邓放明,赵玲艳   

  1. (1.湖南农业大学食品科学技术学院,湖南 长沙 410128;2.湖南农业大学长沙现代食品创新研究院,湖南 长沙 410128)
  • 出版日期:2024-03-25 发布日期:2024-04-03
  • 基金资助:
    国家现代农业产业技术体系建设专项(CARS-24-E-02);湖南农业大学“双一流”建设项目(SYL20182006); 植物源益生菌菌种资源库建设及开发应用研究项目(AU-YJY-B-LX-20-014); 湖南省自然科学基金面上项目(2021JJ30338);发酵辣椒绿色精深加工工程技术研究中心建设项目(2023ZYQ044)

Screening and Identification of Probiotics with in Vitro Anti-Allergic Activity Based on the Degranulation Inhibition of RBL-2H3 Cells

Ma Ding, Qin Shuangxia, Hao Huichao, Deng Fangming, Zhao Lingyan   

  1. (1. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China;2. Changsha Innovation Institute for Food, Hunan Agricultural University, Changsha 410128, China)
  • Online:2024-03-25 Published:2024-04-03

摘要: 为探究乳酸菌的抗过敏活性,本研究采用传统分离技术从发酵蔬菜中获得乳酸菌,并进行益生菌体外评价,结合RBL-2H3细胞脱颗粒抑制实验筛选具有抗过敏活性的益生菌。结果表明,从发酵蔬菜中分离的4 株菌株表现出良好的益生特性和安全性;所选4 株乳酸菌对RBL-2H3细胞脱颗粒释放β-己糖胺酶均具有较高的抑制作用,并对RBL-2H3细胞脱颗粒释放组胺、肿瘤坏死因子-α和白介素-4均有明显的下调作用,具有作为抗过敏活性益生菌的可能性。4 株菌株经16S rDNA测序鉴定为植物乳杆菌2 株、发酵乳杆菌和副干酪乳杆菌各1 株。

关键词: 乳酸菌;RBL-2H3细胞;益生特性;脱颗粒;抗过敏活性

Abstract: To investigate the anti-allergic activity of lactic acid bacteria, lactic acid bacterial strains were obtained from fermented vegetables by the traditional isolation technique and screened for in vitro allergic activity by the degranulation inhibition assay against RBL-2H3 cells. The results showed that all four isolates exhibited good probiotic properties and safety and had a strong inhibitory effect on β-hexosaminidase released from RBL-2H3 cells after degranulation. Moreover, they showed significant down-regulation effects on the release of histamine, tumor necrosis factor-α and interleukin-4 from RBL-2H3 cell after degranulation, suggesting their potential as anti-allergic probiotics. The four strains were identified by 16S rDNA sequencing as two strains of Lactobacillus plantarum, one strain of L. fermentum and one strain of L. paracasei.

Key words: lactic acid bacteria; RBL-2H3 cells; probiotic properties; degranulation; anti-allergic activity

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