不同补料方式对灵芝菌丝体液态深层发酵合成灵芝三萜的影响

摘要/Abstract

摘要： 在50 L发酵罐中采用四种补料培养方式对灵芝菌丝体液态深层发酵合成灵芝三萜进行了比较。实验发现通过补料可以明显地促进灵芝菌丝体的生长和灵芝三萜的合成，同时还发现不同的补料方式对菌丝体生长和灵芝三萜合成有不同的影响：采用指数补料方式可获得较高的菌丝体浓度，并提高了灵芝三萜的含量，与传统的发酵方式相比，采用此补料策略取得了较好的效果。发酵终点灵芝菌丝体干重达到17.68 g/L，灵芝三萜含量达到4.58 g/100 g干菌丝体，分别比分批发酵提高了65.70%和100.88%。

关键词: 灵芝, 深层发酵, 灵芝三萜, 补料培养

Abstract: Four feeding fed-batch culture methods for the production of triterpenes by Ganoderma lucidum G0119 were compared in 50 L fermentor. The results showed that the feeding could obviously promote the mycelium growth of G. lucidum G0119 and the synthesis of triterpenes, and different feeding methods had different influences on the mycelium dry weight and triterpenes content in the broth. The highest mycelical dry weight could obtain by exponential feeding, and the triterpenes content was higher. The results showed that the optimized feeding strategy was the best among all the fermentation strategies, and could obtain the highest mycelical dry weight (17.68 g/L) and triterpenes content (4.58 g/100 g dry mycelium), which was a significant increase (65.70% and 100.88% respectively) compared with a batch process.

Key words: Ganoderma lucidum, Submerged culture, Triterpenes, Fed-batch culture

中图分类号:

TQ920.6

冯杰冯娜杨焱刘方张劲松. 不同补料方式对灵芝菌丝体液态深层发酵合成灵芝三萜的影响[J]. 食品科学, 0, (): 0-0.

参考文献

[1]LIANG S, REN A, MU D S, et al. Current progress in the study on biosynthesis and regulation of ganoderic acids[J]. Applied Microbiology and Biotechnology, 2010, 88(6): 1243-1251. DOI: 10.1007/s00253-010-2871-1.
[2]林志彬. 灵芝的现代研究[M]. 北京: 北京大学医学出版社, 2007: 108-122.
[3]刘高强, 赵艳, 王晓玲, 等. 灵芝多糖的生物合成和发酵调控[J]. 菌物学报, 2011, 30(2): 198-205. DOI: 10.13346/j.mycosystema.2011.02.015.
[4]CHEN S, XU J, LIU C, et al. Genome sequence of the model medicinal mushroom Ganoderma lucidum[J]. Nature Communications, 2012, 3: 913. DOI: 10.1038/ncomms1923.
[5]LIU G Q, XIAO H X, WANG X L, et al. Stimulated production of triterpenoids of Ganoderma lucidum by an ether extract from the medicinal insect, Catharsius molossus and identification of the key stimulating active components[J]. Applied Biochemistry and Biotechnology, 2011, 165(1): 87-97. DOI: 10.1007/s12010-011-9235-x.
[6]XU J W, XU Y N, ZHONG J J. Production of individual ganoderic acids and expression of biosynthetic genes in liquid static and shaking cultures of Ganoderma lucidum[J]. Applied Microbiology and Biotechnology, 2010, 85(4): 941-948. DOI: 10.1007/s00253-009-2106-5.
[7]FENG J, FENG N, YANG Y, et al. Simple and reproducible two-stage agitation speed control strategy for enhanced triterpene production by Lingzhi or Reishi medicinal mushrooms, Ganoderma lucidum ACCC G0119 (Higher Basidiomycetes) based on submerged liquid fermentation[J]. International Journal of Medicinal Mushrooms, 2015, 17(12): 1151-1159.
[8]MU D S, LI C Y, ZHANG X C, et al. Functions of the nicotinamide adenine dinucleotide phosphate oxidase family in Ganoderma lucidum: an essential role in ganoderic acid biosynthesis regulation, hyphal branching, fruiting body development, and oxidative-stress resistance[J]. Environmental Microbiology, 2014, 16(6), 1709-1728. DOI: 10.1111/1462-2920.12326.
[9]REN A, LI X B, MIAO Z G, et al. Transcript and metabolite alterations increase ganoderic acid content in Ganoderma lucidum using acetic acid as an inducer[J]. Biotechnology Letters, 2014, 36(12): 2529-2536. DOI: 10.1007/s10529-014-1636-9.
[10]TANG Y J, ZHANG W, ZHONG J J. Performance analyses of a pH-shift and DOT-shift integrated fed-batch fermentation process for the production of ganoderic acid and ganoderma polysaccharides by medicinal mushroom Ganoderma lucidum[J]. Bioresource Technology, 2009, 100(5): 1852-1859. DOI:10.1016/j.biortech.2008.10.005.
[11]LIU G Q, WANG X L, HAN W J, et al. Improving fermentation production of individual key triterpene, ganoderic acid Me by medicinal fungus Ganoderma lucidum in submerged culture[J]. Molecules, 2012, 17(11): 12575-12586. DOI: 10.3390/molecules171112575.
[12]REN A, LI M J, SHI L, et al. Profiling and quantifying differential gene transcription provide insights into ganoderic acid biosynthesis in Ganoderma lucidum in response to methyl jasmonate[J]. PLoS One, 2013, 8(6): e65027. DOI: 10.1371/journal.pone.0065027.
[13]ZHONG J J, XU Y N, TAN G Y, et al. Signal transduction engineering: a powerful platform technology for enhancing secondary metabolite production[J]. New Biotechnology, 2014, 31: S23-S24. DOI: 10.1016/j.nbt.2014.05.1666.
[14]ZHANG J, ZHONG J J, GENG A. Improvement of ganoderic acid production by fermentation of Ganoderma lucidum with cellulase as an elicitor[J]. Process Biochemistry, 2014, 49(10): 1580-1586. DOI: 10.1016/j.procbio.2014.06.018.
[15]DING S F, TAN T W. L-lactic acid production by Lactobacillus casei fermentation using different fed-batch feeding strategies[J]. Process Biochemistry, 2006, 41: 1451-1454. DOI: 10.1016/j.procbio.2006.01.014.
[16]冯杰, 冯娜, 贾薇, 等. 灵芝菌丝体液态深层发酵合成灵芝三萜动力学研究[J]. 食用菌学报, 2015, 22(3): 74-79. DOI: 10.16488/j.cnki.1005-9873.2015.03.016.
[17]冯杰, 冯娜, 杨焱, 等. 通气量对灵芝菌丝体液态深层发酵合成灵芝三萜的影响[J]. 天然产物研究与开发, 2015, 27(9): 1564-1570. DOI: 10.16333/j.1001-6880.2015.09.010.
[18]FENG J, ZHANG J S, JIA W, et al. An unstructured kinetic model for the improvement of triterpenes production by ganoderma lucidum G0119 based on nitrogen source effect[J]. Biotechnology and Bioprocess Engineering, 2014, 19(4): 727-732. DOI: 10.1007/s12257-014-0049-x.
[19]HE L, XU Y Q, ZHANG X H. Medium factor optimization and fermentation kinetics for phenazine-1-carboxylic acid production by Pseudomonas sp. M18G[J]. Biotechnology and Bioengineering, 2008, 100: 250-259. DOI: 10.1002/bit.21767.
[20]FANG Q H, ZHONG J J. Two-stage culture process for improved production of ganoderic acid by liquid fermentation of higher fungus Ganoderman lucidum[J]. Biotechnology Progress, 2002, 18: 51-54. DOI: 10.1021/bp010136g.
[21]XU Y N, XIA X X, ZHONG J J. Induction of ganoderic acid biosynthesis by Mn2+ in static liquid cultivation of Ganoderma lucidum[J]. Biotechnology and Bioengineering, 2014, 111(11): 2358-2365. DOI: 10.1002/bit.25288.
[22]XU Y N, XIA X X, ZHONG J J. Induced effect of Na+ on ganoderic acid biosynthesis in static liquid culture of Ganoderma lucidum via calcineurin signal transduction[J]. Biotechnology and Bioengineering, 2013, 110(7): 1913-1923. DOI: 10.1002/bit.24852.