食品科学 ›› 2020, Vol. 41 ›› Issue (15): 134-139.doi: 10.7506/spkx1002-6630-20190725-337

• 食品工程 • 上一篇    下一篇

超声作用下虎乳灵芝多糖-硒纳米粒子的非酶糖基化抑制作用

肖益东,蔡文斐,郑赵敏,马会雨,黄琪琳   

  1. (1.华中农业大学食品科学技术学院,湖北 武汉 430070;2.环境食品学教育部重点实验室,湖北 武汉 430070)
  • 出版日期:2020-08-15 发布日期:2020-08-19
  • 基金资助:
    国家自然科学基金面上项目(31771996)

Inhibition Effect of Lignosus rhinocerotis Polysaccharides-Selenium Nanoparticles Prepared by Ultrasound Treatment on Non-enzymatic Glycosylation

XIAO Yidong, CAI Wenfei, ZHENG Zhaomin, MA Huiyu, HUANG Qilin   

  1. (1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;2. Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan 430070, China)
  • Online:2020-08-15 Published:2020-08-19

摘要: 为探究虎乳灵芝多糖-硒纳米粒子(Lignosus rhinocerotis polysaccharides-selenium nanoparticles,LRP-SeNPs)对非酶糖基化反应的抑制作用,在超声处理下,制备稳定的LRP-SeNPs,运用紫外光谱、荧光光谱和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE)法研究LRP-SeNPs对非酶糖基化反应3 个阶段的特征产物(果糖胺、二羰基化合物及荧光终产物)的影响。结果表明,超声处理的虎乳灵芝多糖-硒纳米粒子(ultrasound treated LRP-SeNPs,U-LRP-SeNPs)能有效抑制非酶糖基化反应,且存在一定的剂量依赖效应;其中U-LRP-SeNPs=1∶10、1∶15(Se/LRP质量比)具有一定的非酶糖基化抑制活性,抑制率分别保持在30%和20%;进一步通过SDS-PAGE验证U-LRP-SeNPs的抑制作用。超声有效提高了LRP-SeNPs的稳定性,使硒纳米粒子尺寸变小,比表面积变大,从而提供更多与自由基结合的位点,屏蔽或终止了自由基,有效抑制自由基引发的氧化反应,将非酶糖基化反应阻止于氧化阶段。

关键词: 虎乳灵芝多糖-硒纳米粒子, 超声, 非酶糖基化, 抑制作用

Abstract: In order to explore the inhibitory effect of Lignosus rhinocerotis polysaccharides-selenium nanoparticles (LRP-SeNPs) on the non-enzymatic glycosylation reaction, stable LRP-SeNPs were synthesized under ultrasound irradiation, and the effects of LRP-SeNPs on the characteristic products (fructosamine, dicarbonyl compounds and fluorescence end products) of non-enzymatic glycosylation reaction at three stages were studied by ultraviolet spectroscopy, fluorescence spectroscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that ultrasound treated LRP-SeNPs (U-LRP-SeNPs) could effectively inhibit non-enzymatic glycosylation reaction in a dose-dependent manner. The percentage inhibition of non-enzymatic glycosylation by U-LRP-SeNPs with Se/LRP ratio of 1:10 and 1:15 (m/m) remained at 30% and 20%, respectively. The inhibitory effect of U-LRP-SeNPs was further verified by SDS-PAGE. Ultrasound could effectively improve the stability of LRP-SeNPs, reduce the size of SeNPs, and increase the specific surface area, thereby providing more sites for binding with free radicals to shield or terminate free radicals, effectively inhibit oxidation reaction caused by free radicals, and finally prevent non-enzymatic glycation reaction at the oxidation stage.

Key words: Lignosus rhinocerotis polysaccharides-selenium nanoparticles (LRP-SeNPs), ultrasound, non-enzymatic glycosylation, inhibition effect

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