食品科学 ›› 2011, Vol. 32 ›› Issue (13): 188-193.doi: 10.7506/spkx1002-6630-201113041

• 生物工程 • 上一篇    下一篇

不同发酵条件对Fusarium. solani ZH0101产木聚糖酶的影响

王权帅,康文丽,生吉萍,申琳*   

  1. 中国农业大学食品科学与营养工程学院
  • 出版日期:2011-07-15 发布日期:2011-07-02
  • 基金资助:
    国家公益性行业(农业)科研专项经费资助项目(200803033)

Optimization of Fermentation Condition for Xylanase Production by Fusarium solani ZH0101

WANG Quan-shuai,KANG Wen-li,SHENG Ji-ping,SHEN Lin*   

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Online:2011-07-15 Published:2011-07-02

摘要: 以本实验室筛选到的一株产木聚糖酶但不产纤维素酶的真菌Fusarium solani ZH0101为供试菌株,利用麦秸为诱导物,对产木聚糖酶的液体发酵培养基进行优化。对发酵时间、麦秸添加量、培养基起始pH值、磷酸盐、金属离子、无机氮源和有机氮源对产酶的影响进行研究,优化最佳的培养条件。优化后的液体发酵培养基条件为:麦秸20g/L、KH2PO4 4g/L、CH3COONH4 1g/L、酵母膏2g/L和起始pH值为6.0,发酵时间为14d。优化条件下所产无纤维素酶活力的木聚糖酶酶活力为26.85U/mL,能比未优化前的20.82U/mL提高近30%。

关键词: 液体发酵, Fusarium solani, 木聚糖酶

Abstract: Cellulase-free xylanase has been widely applied in the food industry as well as the paper and pulp industry. Wheat straw as one of the most important agricultural by-products can be used to induce cellulase-free xylanase production through fermentation. In the present study, the optimal conditions of wheat straw fermentation by Fusarium solani ZH0101 for producing cellulase-free xylanase were 20 g/L wheat straw, 4 g/L KH2PO4, 1 g/L CH3COONH4, 2 g/L yeast extract, initial pH of 6.0 and fermentation time of 14 days. Under the optimal fermentation conditions, the cellulase-free xylanase activity could reach up to 26.85 U/mL, which exhibited a 30% enhancement compared with the fermentation in original medium.

Key words: Fusarium solani ZH0101, xylanase, submerged fermentation

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