食品科学 ›› 2011, Vol. 32 ›› Issue (14): 25-28.doi: 10.7506/spkx1002-6630-201114006

• 工艺技术 • 上一篇    下一篇

响应面法优化中性蛋白酶提取牡蛎牛磺酸酶解工艺条件

刘亚南,张志胜*,佟海菊,孙克岩,宋 欣   

  1. 河北农业大学食品科技学院
  • 出版日期:2011-07-25 发布日期:2011-06-18
  • 基金资助:
    国家海洋公益性行业科研专项(200805046)

Employing Response Surface Methodology to Optimize Process Conditions for Enzymatic Extraction of Taurine from Oyster Meat with Neutral Protease

LIU Ya-nan,ZHANG Zhi-sheng*,TONG Hai-ju,SUN Ke-yan,SONG Xin   

  1. (College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China)
  • Online:2011-07-25 Published:2011-06-18

摘要: 利用中性蛋白酶对牡蛎进行酶解,在单因素试验基础上,研究加酶量、pH值、温度3个因素对酶解过程中牛磺酸提取量的影响,通过三因素三水平Box-Behnken响应面分析法优化其酶解工艺。结果显示:在加酶量1300U/g、pH7.5、温度48℃条件下,牛磺酸提取量实际值可达2.724mg/g。

关键词: 牡蛎, 牛磺酸, 响应面法, 酶解

Abstract: In this study, neutral protease hydrolysis was used to extract taurine from oyster meat and the extraction process was optimized. On the basis of one-factor-at-a-time experiments, a three-variable, three-level Box-Behnken experimental design coupled with response surface analysis was employed to explore the effects of enzyme dosage, pH and temperature on the extraction efficiency of taurine. The optimum hydrolysis conditions for taurine extraction were enzyme dosage 1300 U/g fresh meat, pH 7.5 and hydrolysis temperature 48 ℃, and the resulting extraction yield of taurine was 2.724 mg/g fresh meat.

Key words: oyster, taurine, response surface methodology, enzymolysis

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