IKnife质谱实时采集南极犬牙鱼脂质组学轮廓技术研究

摘要/Abstract

摘要： 快速蒸发离子化质谱（Rapid evaporative ionization mass spectrometry, REIMS）是一种无须样品制备和液相色谱分离的新型原位电离质谱技术，通过iKnife手持采样装置电切生物组织样品，即可实现化合物离子化，并由离子管路传输至质量分析器进行实时检测的技术。本研究基于该技术对低脂鱼类南极犬牙鱼的肌肉组织进行脂质组学轮廓检测，并通过响应面（Box-Benhnken）分析优化了系统参数，包括能量25 W、切割速度0.50 mm/s、切割长度1.0 cm。在最佳实验条件下共检出脂肪酸离子17种，其中信号响应强度最大的为m/z 327.23，相对含量达到了17.81%，经鉴定其结构为FA 22:6，其次分别为m/z 255.23（FA 16:0，9.81%），m/z 281.25（FA 18:1，9.09%）；检出磷脂离子10种，质量范围m/z 736.49~909.55，信号最强离子峰m/z 885.55经鉴定为[PI 38:4-H]–，相对丰度达到了19.30%，其次为m/z 790.54（[PE 40:6-H]–，15.24%）。方法验证结果显示，目标离子的信噪比为35.4-95.3，质量偏差为4.17-9.78 ppm，日内精密度RSD为3.7-5.6%，日间精密度RSD为5.9-7.3%，本实验方法灵敏度和分辨率高、检测速度快、结果稳定，为脂质组学研究和鱼类生物信息检测提供了新的技术手段。

关键词: 快速蒸发离子化质谱, iKnife, 脂质组学, 南极犬牙鱼

Abstract: Rapid evaporative ionization mass spectrometry (Rapid evaporative ionization mass spectrometry, REIMS) is a kind of novel ambient mass spectrometry without any sample preparation and liquid chromatographic separation processes.The compounds from biological samples could be easily ionized by electric cutting of the surface using an iKnife setup. The ions generated was transferred through the ion transfer tube and analyzed by the mass spectrometer. Based on this technique, the tissue of low-fat fish, Dissostichus eleginoides, was analyzed by lipidomics profiling. The instrumental parameters were optimized such that the power output was 25 W, the cutting speed was 0.50 mm/s, and the cutting length was 1.0 cm, using response surface methodology (Box-Benhnken). Under the optimized conditions, a total of 17 fatty acid molecular species was detected, among which the ion of m/z 327.23 (FA 22:6, 17.81%) was the most significant, followed by the m/z 255.23 (FA 16:0, 9.81%), and m/z 281.25 (FA 18:1, 9.09%). A total of 10 phospholipid molecular species was tentatively identified in the m/z range from 736.49 to 909.55. The peak at m/z 885.55 was the most intensive peak with relative content 19.30%, which was identified as [PI 38:4-H]–, followed by the m/z 790.54 ([PE 40:6-H]–, 15.24%). The method was validated and the results showed that the signal-to-noise ratios of the target ions were 35.4-95.3, with error values 4.17-9.78 ppm, The RSDs of intra-day precision and inter-day precision were 3.7-5.6% and 5.9-7.3%, respectively. The results indicated that this method is sensitive, the resolution is high, and the result is stable. It provides a totally new technology and platform for lipidomics and bioinformations of fish.

Key words: Rapid evaporative ionization mass spectrometry (REIMS), IKnife, Lipidomics, Dissostichus eleginoides

中图分类号:

TS254.1

陈康王海星张燕平饶伟沈清. IKnife质谱实时采集南极犬牙鱼脂质组学轮廓技术研究[J]. 食品科学, 0, (): 0-0.

Kang Chen Yan-Ping ZHANG Qing SHEN. IKnife Monopoloar Electrode Coupling Rapid Evaporative Ionization Mass Spectrometry for Lipidomics Profiling of Patagonian Toothfish (Dissostichus eleginoides)[J]. FOOD SCIENCE, 0, (): 0-0.

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