关键词: 竹荪多糖, 菌丝体, 发酵工艺, 优化, 规模化生产

Abstract: The main purpose of this study was to increase the intracellular polysaccharide yield of Dictyophora indusiata mycelia using a large-scale production process. The optimum conditions for the submerged fermentation of D. indusiata mycelia were determined by the screening of the components, the single factor experiment and the Box-Behnken designs method: glucose 32.3 g/L, yeast powder 4.5 g/L, potassium dihydrogen phosphate 2.1 g/L, and magnesium sulfate 2.5 g/L. Under this condition, the predicted value of intracellular polysaccharide yield was 1.23 g/L. Shake flask experiments confirmed that the intracellular polysaccharide yield was 1.19 g/L, which was equivalent to the predicted value. The yields of intracellular polysaccharides in the 5 L, 30 L and 50 L fermentors were 1.43 g/L, 1.28 g/L and 1.26 g/L, respectively, which were higher than the predicted values of 16.26%, 4.06% and 2.43%, respectively. The liquid submerged fermentation process of D. indusiata mycelium obtained from this study has effectively promoted the high yield of intracellular polysaccharides of D. indusiata. In this study, the liquid submerged fermentation process of D. indusiata mycelium optimized effectively promoted the high yield of intracellular polysaccharides. This process can provide a good reference for the large-scale production and application of D. indusiata.

Key words: Dictyophora indusiata polysaccharide, Mycelium, Fermentation process, Optimization, Large-scale production